Services
Indigo Biosciences specializes in screening of small molecules against nuclear receptors (NRs) and other drug targets. We offer profiling of effects of compounds on gene expression using reporter genes, microarrays, and real-time PCR. We can rapidly determine agonist EC50 values or antagonist IC50 values of nuclear receptor ligands using luciferase-based whole-cell assays. We further offer functional bioassays and custom bioassay development using state-of-the-art techniques involving biochemistry, molecular / cellular biology, and synthetic molecular probes. Please click on any category for more information.
We offer three panels for testing of individual compounds against multiple targets: Metabolic Disease (Cholesterol and Lipid Homeostasis), Toxicology (Drug Metabolism and Transport), and Endocrinology (Steroid Receptors).
Gene expression analysis by experts.
Our experienced research team will work with you to create high throughput reporter gene assays, binding assays or biomarker analysis for any target or disease.
Our current portfolio includes assays for 18 human NRs.
Quantitate changes in the expression of mRNA for genes of interest.
Cytotoxicity and proliferation assays; adipocyte differentiation and adipogenesis marker analysis; C-reactive protein reporter assays; fluorescence polarization assays, and more.