P53 is frequently referred to as the “guardian of the genome” due to its ability to limit the accumulation of DNA damage by responding to cellular stress and as a regulator of the cell cycle. When activated by factors such as ionizing radiation and other mutagens, hypoxia, and nutrient deprivation, P53 can regulate genes that signal a cell to undergo apoptosis (cell death), senescence (cessation of division), or DNA repair. In this way, it helps to protect the genome and prevent formation of cancer.
INDIGO’s P53 assay kit is all-inclusive, offering everything needed to perform the assay to quantify any functional activity that a compound may exert against human P53. This includes P53 Reporter Cells, optimized media for use during cell culture and in diluting test samples, a reference P53 activator, Luciferase Detection Reagent, two cell culture-ready assay plates, and a detailed protocol. This allows researchers to obtain results quickly since there is no need to procure components from multiple sources or to transfect and propagate cells.
INDIGO’s assay kit is available in a 2 x 48 format allowing researchers to screen small numbers of test compounds as well as custom bulk reagents for high throughput screening applications. INDIGO can also perform the assay in its own lab as a service for researchers.