Liver X Receptors Panel (LXRα, LXRβ)
| Product Family | Product Number | Product Description | Technical Manual |
| Liver X Receptor (LXRα, LXRβ) |
IB00321-48P | Human Liver X Receptor Panel (LXRα, LXRβ), 2x 48-well assays |
Technical Manual |
Liver X Receptor Assay Panel Background
Liver X receptor α (LXR-α or LXRα) is a nuclear receptor protein encoded by the NR1H3 gene (nuclear receptor subfamily 1, group H, member 3). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
Liver X receptor β (LXR-β or LXRβ) is a member of the nuclear receptor family of transcription factors. LXRβ is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
This Liver X Receptor PANEL of assays utilizes non-human mammalian cells engineered to express the human LXR proteins: LXRα (NR1H3) and LXRβ (NR1H2).
Liver X Receptor Assay Panel Kit
This Liver X Receptor PANEL assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to reporter cells for each LXR, optimized growth media, media for diluting test compounds, a control agonist for each LXR, luciferase detection reagent, a cell culture-ready assay plate in strip-well format so that (if preferred) LXRα and LXRβ assays may be performed at different times, detailed protocol, and a protocol quick guide.
This PANEL contains sufficient materials to perform 48 LXRα assays and 48 LXRβ assays, all in a single 96-well plate format.
LXR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
Liver X Receptor Assay Panel Services
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
Liver X Receptor Assay Panel Research Areas
Diabetes; Dyslipidemia; Artherosclerosis; CNS, Circadian and Basal Metabolism; Lipid Metabolism and Energy; Metabolic Disease; NASH/NAFLD