Liver X Receptor Panel (LXRα, LXRβ)
This Liver X Receptor PANEL of assays utilizes non-human mammalian cells engineered to express the human LXR proteins: LXRα (NR1H3) and LXRβ (NR1H2).
Liver X receptor α (LXR-α or LXRα) is a nuclear receptor protein encoded by the NR1H3 gene (nuclear receptor subfamily 1, group H, member 3). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
Liver X receptor β (LXR-β or LXRβ) is a member of the nuclear receptor family of transcription factors. LXRβ is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with retinoid X receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
This PANEL contains sufficient materials to perform 48 LXRα assays and 48 LXRβ assays, all in a single 96-well plate format.
LXR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
The assay system is all inclusive, providing reporter cells for each LXR, optimized growth media, media for diluting test compounds, a control agonist for each LXR, luciferase detection reagent, a cell culture-ready assay plate in strip-well format so that (if preferred) LXRα and LXRβ assays may be performed at different times, detailed protocol, and a protocol quick guide. The kit is shipped on dry ice.
| Product Description | Product Number | |
|---|---|---|
| Human LXR Reporter Assays PANEL | IB00321-48P |
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters. To initiate a Service Study, download and complete all fields of the Excel worksheet “Service Work Order" then submit the electronic file to INDIGO Customer Service.
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