Peroxisome Proliferator-Activated Receptors Panel (PPARα, PPARδ, PPARγ)
|Product Family||Product Number||Product Description||Technical Manual|
|Peroxisome Proliferator-Activated Receptor||IB00131-32P||Human Peroxisome Proliferator-Activated Receptor Panel (PPARα, PPARδ, PPARγ), 3x 32-well assays||Technical Manual|
This PANEL of PPAR assays is an all inclusive assay system that includes in addition to reporter cells for each PPAR, optimized growth media, media for diluting test compounds, a control agonist for each PPAR, luciferase detection reagent, detailed protocol, and a protocol quick guide, and a cell culture-ready assay plate in strip-well format so that (if preferred) PPARα, PPARδ, and PPARγ assays may be performed at different times.
This PANEL contains sufficient materials to perform 32 PPARα assays, 32 PPARγ assays, and 32 PPARδ assays, all in a single 96-well plate format.
PPAR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
PPAR ligands, whose names derived from their effect on inducing an increase in the size and number of peroxisomes, include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified.
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.