Retinoid X Receptors Panel (RXRα, RXRβ, RXRγ)
|Product Family||Product Number||Product Description||Technical Manual|
|Retinoid X Receptor||IB00831-32P||Human Retinoid X Receptor Panel
(RXRα, RXRβ, RXRγ), 3x 32-well assays
This PANEL of Retinoid X Receptors Reporter Assays is all inclusive assay system which includes in addition to reporter cells for each RXR, optimized cell culture medium, a medium for diluting test compounds, a control agonist for each RXR, stable-glow luciferase detection reagent, detailed protocol, Protocol Quick Guide, and a cell culture-ready assay plate in strip-well format so that (if preferred) RXRα, RXRβ, and RXRγ assays may be performed at different times.
This PANEL contains sufficient materials to perform 32 RXRα assays, 32 RXRβ assays, and 32 RXRγ assays in 96-well plate format.
RXR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcription factors.
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.