Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Rodent (Mouse/Rat) Peroxisome Proliferator-Activated Receptor gamma (mrPPARg). INDIGO’s mrPPARg reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the mrPPARg. In addition to mrPPARg Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against mrPPARg. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Features
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
Product Specifications
Target Type | Nuclear Hormone Receptor, Nuclear Receptor Orthologs | ||
Species | Rodent (Mouse / Rat shared ortholog) | ||
Receptor Form | Hybrid | ||
Assay Mode | Agonist, Antagonist | ||
Kit Components |
| ||
Shelf Life | 6 months | ||
Shipping Requirements | Dry Ice | ||
Storage temperature | -80C |
Data
Target Background
Peroxisome proliferator-activated receptor gamma (PPAR-gamma or PPARγ), also known as the glitazone receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3) is a type II nuclear receptor that in humans is encoded by the PPARG gene. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. PPARγ regulates adipocyte differentiation, fatty acid storage and glucose metabolism. The PPARγ knockout mice fail to generate adipose tissue when fed a high fat diet. Many insulin sensitizing drugs used in the treatment of diabetes target PPARγ as a means to lower serum glucose without increasing pancreatic insulin secretion. Additionally, PPARγ has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
INDIGO’s Mouse/Rat PPAR gamma (mrpparγ; nr1c3) Assay utilizes proprietary mammalian cells engineered to provide high-level expression of mrPPARγ. Reporter Cells also incorporate an mrPPARγ-responsive luciferase reporter gene, therefore, quantifying expressed luciferase activity provides a sensitive surrogate measure of mrPPARγ activity in the treated cells. The principle application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against mrPPARγ.
Citations
Also available as a service