Peroxisome proliferator-activated receptor (PPAR) agonists are known to modulate the synthesis of dermal lipids and proteins including collagens. Olive (Olea europaea) leaves have been reported to contain PPAR-binding ligands. Collagen IV, a major dermal-epidermal junction (DEJ) protein, degrades with both age and disease. Here, we report the formulation of a novel multi-ligand complex, Linefade, and its effects on collagen IV synthesis.
Linefade prepared from the leaves of Olea europaea contains 2% w/w plant extract solids dissolved in a mixture of glyceryl monoricinoleate and dimethyl isosorbide. In silico docking was performed with PPAR-α (PDB ID: 2P54). Linefade was evaluated for PPAR-α-dependent transcription in a luciferase reporter assay system. Cell viability and collagen IV levels in human dermal fibroblasts cultures were measured using the MTT method and ELISA assay, respectively. Transcriptome analysis was conducted on a full-thickness reconstituted human skin (EpiDermFT) model. Ex vivo cell viability and collagen IV immunostaining were performed on human skin explants.
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Date of publication: 18 October 2021; International Journal of Cosmetic Science
Author information: George P. Majewski (1), Smrita Singh (2), Krzysztof Bojanowski (3)
(1) Contrast Product Development, 340 S Lemon Ave, #6006, Walnut, 91789
(2) Creative Bioinformatics and Science, Morna, District Bijnor, Uttar Pradesh, India, 246761
(3) Sunny BioDiscovery, Santa Paula, 93060