• PRODUCTS
    • Assay Kit Platform & Formats
    • All Receptor Assays
    • Environmental Monitoring Assays
    • Growth Factor Assays
    • Ortholog Receptor Assays
    • Toxicology Assays
      • In Vitro Hepatotoxicity Assay Kit
      • Gene Expression Profiling Assay Kit
      • P-gp/MDR1 Drug Interaction Assay Kit
    • INDIGlo Luciferase Detection Reagent
    • Live Cell Multiplex
  • SERVICES
    • Reporter Assay Services
    • Nuclear Hormone Receptor Profiling
    • Environmental Monitoring Services
    • Gene Expression Profiling Services
    • In Vitro Hepatotoxicity Screening Services
    • Custom Assay Development
  • APPLICATIONS
    • Drug Discovery
    • Discovery Toxicology
    • Disease States
      • Overview
      • Anemia & Kidney Disease
      • Autoimmune Disease & Inflammation
      • Cancer
      • Cardiovascular Disease
      • Diabetes
      • Endocrinology
      • NAFLD/NASH
      • Obesity
    • Environmental Monitoring Applications
  • RESOURCES
    • Nuclear Receptor Overview
    • Webinars and Publications
      • New Research Publications
      • Webinars from INDIGO
      • Scientific Whitepapers from INDIGO
      • Scientific Posters from INDIGO
      • BLOG
    • Product Literature
      • INDIGO Technical Manuals
      • Product SDS
      • upcyte® Hepatocytes
    • FAQ
  • ABOUT
    • About INDIGO
    • Why INDIGO
    • Key Personnel
      • Board of Directors & Advisors
      • Management
    • Employment
    • INDIGO Press Releases
    • INDIGO in the News
  • CONTACT
    • Contact INDIGO
    • Request a Quote
    • Ordering & Distributors
Search site...

± α β γ δ Δ ε ζ κ ω ö ® ™ µ

  • PRODUCTS
    • Assay Kit Platform & Formats
    • All Receptor Assays
    • Environmental Monitoring Assays
    • Growth Factor Assays
    • Ortholog Receptor Assays
    • Toxicology Assays
      • In Vitro Hepatotoxicity Assay Kit
      • Gene Expression Profiling Assay Kit
      • P-gp/MDR1 Drug Interaction Assay Kit
    • INDIGlo Luciferase Detection Reagent
    • Live Cell Multiplex
  • SERVICES
    • Reporter Assay Services
    • Nuclear Hormone Receptor Profiling
    • Environmental Monitoring Services
    • Gene Expression Profiling Services
    • In Vitro Hepatotoxicity Screening Services
    • Custom Assay Development
  • APPLICATIONS
    • Drug Discovery
    • Discovery Toxicology
    • Disease States
      • Overview
      • Anemia & Kidney Disease
      • Autoimmune Disease & Inflammation
      • Cancer
      • Cardiovascular Disease
      • Diabetes
      • Endocrinology
      • NAFLD/NASH
      • Obesity
    • Environmental Monitoring Applications
  • RESOURCES
    • Nuclear Receptor Overview
    • Webinars and Publications
      • New Research Publications
      • Webinars from INDIGO
      • Scientific Whitepapers from INDIGO
      • Scientific Posters from INDIGO
      • BLOG
    • Product Literature
      • INDIGO Technical Manuals
      • Product SDS
      • upcyte® Hepatocytes
    • FAQ
  • ABOUT
    • About INDIGO
    • Why INDIGO
    • Key Personnel
      • Board of Directors & Advisors
      • Management
    • Employment
    • INDIGO Press Releases
    • INDIGO in the News
  • CONTACT
    • Contact INDIGO
    • Request a Quote
    • Ordering & Distributors

Olive Leaf-Derived PPAR Agonist Complex Induces Collagen IV Synthesis in Human Skin Models

Print Friendly, PDF & Email

Abstract

Introduction
Peroxisome proliferator-activated receptor (PPAR) agonists are known to modulate the synthesis of dermal lipids and proteins including collagens. Olive (Olea europaea) leaves have been reported to contain PPAR-binding ligands. Collagen IV, a major dermal-epidermal junction (DEJ) protein, degrades with both age and disease. Here, we report the formulation of a novel multi-ligand complex, Linefade, and its effects on collagen IV synthesis.

Methods
Linefade prepared from the leaves of Olea europaea contains 2% w/w plant extract solids dissolved in a mixture of glyceryl monoricinoleate and dimethyl isosorbide. In silico docking was performed with PPAR-α (PDB ID: 2P54). Linefade was evaluated for PPAR-α-dependent transcription in a luciferase reporter assay system. Cell viability and collagen IV levels in human dermal fibroblasts cultures were measured using the MTT method and ELISA assay, respectively. Transcriptome analysis was conducted on a full-thickness reconstituted human skin (EpiDermFT) model. Ex vivo cell viability and collagen IV immunostaining were performed on human skin explants.

To read the full publication, click here.

Date of publication: 18 October 2021; International Journal of Cosmetic Science

Author information: George P. Majewski (1), Smrita Singh (2), Krzysztof Bojanowski (3)

 

(1) Contrast Product Development, 340 S Lemon Ave, #6006, Walnut, 91789
(2) Creative Bioinformatics and Science, Morna, District Bijnor, Uttar Pradesh, India, 246761
(3) Sunny BioDiscovery, Santa Paula, 93060

 

Search site...
Click to Insert Symbols in Search

α β γ δ Δ ε ζ κ ® ™ µ

Request a Quote

Want More Information?

Resource Quick Links

  • Technical Manuals & Product Listing
  • Safety Data Sheets
  • Sample Study Report
  • Study Work Order Form

3006 Research Drive, Suite A1, State College, PA, USA 16801

+1 (814) 234-1919

  • Terms Conditions
  • Privacy Policy
  • Product Policies
  • Limited Use Disclosure

© 2022 INDIGO Biosciences, Inc. All Rights Reserved