Mouse Pregnane X Receptor (mPXR; nr1i2)
|Product Family||Product Number||Product Description||Technical Manual|
|M07001-32||Mouse PXR Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|M07001||Mouse PXR Reporter Assay System, 1 x 96-well format assays||Technical Manual|
Mouse Pregnane X Receptor Assay Kit
This Mouse Pregnane X Receptor (mPXR) assay kit is an all-inclusive firefly luciferase reporter assay system that includes, in addition to mPXR Reporter Cells, two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. mPXR Reporter Cells cannot be refrozen or maintained in extended culture.
mPXR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
PXR assay kits also available in: Human, Rat, Cynomolgus Monkey, Dog
Mouse Pregnane X Receptor Assay Service
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and “vehicle” control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
PXR Service Assays: Human, Rat, Mouse, Cynomolgus Monkey, Dog
Mouse Pregnane X Receptor Assay Background
Pregnane X Receptor also known as nuclear receptor subfamily 1, group I, member 2 (NR1I2) and SXR is a nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. PXR activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs, and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones, its response to specific ligands is species-specific. PXR is activated by naturally occurring steroids, such as pregnenolone and progesterone and binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
This assay product utilizes proprietary mammalian cells engineered to provide constitutive, high-level expression of the Mouse Pregnane X Receptor (nr1i2), a ligand-dependent transcription factor commonly referred to as PXR. PXR is also known as the Steroid and Xenobiotic sensing nuclear receptor (SXR).
For more information on PXR, visit the Nuclear Receptor Resource.