Rat RAR-related Orphan Receptor Gamma (rRORγ; nr1f3)
|Product Family||Product Number||Product Description||Technical Manual|
|R04001-32||Rat RORγ Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|R04001||Rat RORγ Reporter Assay System, 1 x 96-well format assays||Technical Manual|
Rat RAR-related Orphan Receptor Gamma Assay Kit
This Rat RAR-related orphan receptor gamma (rRORγ) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to rRORγ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
rRORγ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
RORg assay kits also available in Human
Rat RAR-related Orphan Receptor Gamma Assay Services
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
RORg Service Assays: Human, Mouse
Rat RAR-related Orphan Receptor Gamma Assay Background
RAR-related orphan receptor gamma (ROR-gamma), also known as NR1F3 (nuclear receptor subfamily 1, group F, member 3) is a nuclear receptor encoded by the RORG gene. The RAR-related orphan receptors (RORs) are members of the nuclear receptor family of intracellular transcription factors. There are three forms of ROR, ROR-α, -β, and -γ and each is encoded by a separate gene (RORA, RORB, and RORC respectively). The RORs are somewhat unusual in that they appear to bind as monomers to hormone response elements as opposed to the majority of other nuclear receptors which bind as dimers. Melatonin has been reported to be the endogenous ligand for ROR-α while CGP 52608 has been identified as a ROR-α selective synthetic ligand. However X-ray crystallographic (PDB 1n83 and 1s0x) and functional data both suggest that cholesterol or a cholesterol derivative may be the endogenous ligand. In contrast, all-trans retinoic acid binds with high affinity to ROR-β and -γ but not ROR-α.
INDIGO’s Rat RORγ (RAR-related Orphan Receptor Gamma; nr1f3) referred to as rat rorC, or here as rRORγ. The N-terminal DNA binding domain (DBD) of the native rRORγ receptor has been substituted with that of the yeast GAL4-DBD. The principle application of this reporter assay is in the screening of test samples to quantify inverse-agonist or agonist activities that they may exert against rat RORγ.
For more information on RORγ, visit the Nuclear Receptor Resource.