Zebrafish Glucocorticoid Receptor (zGR; nr3c1)
|Product Family||Product Number||Product Description||Technical Manual|
|Z00201-32||Zebrafish GR Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|Z00201||Zebrafish GR Reporter Assay System, 1 x 96-well format assays||Technical Manual|
This Zebrafish Glucocorticoid Receptor (zGR) kit product is an all-inclusive assay system that includes, in addition to zGR Reporter Cells, two optimized media for use during cell culture and in diluting the user's test samples, the reference agonist Dexamethasone, Luciferase Detection Reagent, and a cell culture-ready assay plate.
Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
INDIGO’s Zebrafish Glucocorticoid Receptor (nr3c1) nuclear receptor assay utilizes proprietary mammalian cells engineered to provide constitutive high level expression of full-length, unmodified Zebrafish (Danio rerio) Glucocorticoid Receptor (nr3c1), a ligand-dependent transcription factor, commonly referred to as GR.
INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in zfGR activity. Luciferase gene expression occurs after ligand-bound zfGR undergoes DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO's reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo
For more information on GR, visit the Nuclear Receptor Resource.
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.