Rat Peroxisome Proliferator-Activated Receptor Alpha (rPPARα; nr1c1)
|Product Family||Product Number||Product Description||Technical Manual|
|R00111-32||Rat PPARα Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|R00111||Rat PPARα Reporter Assay System, 1 x 96-well format assays||Technical Manual|
Rat Peroxisome Proliferator-Activated Receptor Alpha Assay Kit
This Rat Peroxisome Proliferator-Activated Receptor Alpha (rPPARα) assay kit is an all-inclusive firefly luciferase reporter assay system that includes, in addition to rPPARα Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
rPPARα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96-well assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
PPARa assay kits also available in: Human, Mouse, Cyn Monkey, Dog
Rat Peroxisome Proliferator-Activated Receptor Alpha Assay Services
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
PPARa Service Assays: Human, Mouse, Rat, Cyn Monkey, Dog
Rat Peroxisome Proliferator-Activated Receptor Alpha Assay Background
INDIGO’s Rat PPAR alpha (nr1c1; pparA; rPPARα) Assay utilizes proprietary rodent cells engineered to provide high-level expression of rPPARα. Reporter Cells also incorporate a responsive luciferase reporter gene, therefore, quantifying expressed luciferase activity provides a sensitive surrogate measure of rPPARα activity in the treated cells.
The principle application of this reporter assay system is in the screening of test samples to quantify functional activity, either agonist or antagonist, that they may exert against rat PPARα.
For more information on PPARα, visit the Nuclear Receptor Resource.