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Zebrafish AhR Reporter Assay Kit
Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Zebrafish Aryl Hydrocarbon Receptor. INDIGO’s zAhR reporter assay utilizes proprietary non-human cells that have been engineered to provide constitutive expression of the zAhR. In addition to zAhR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against zAhR. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Features
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
Product Specifications
| Target Type | Nuclear Hormone Receptor, Nuclear Receptor Orthologs | ||
| Species | Zebrafish | ||
| Receptor Form | Native | ||
| Assay Mode | Agonist, Antagonist | ||
| Kit Components |
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| Shelf Life | 6 months | ||
| Shipping Requirements | Dry Ice | ||
| Storage temperature | -80C |
Data
Target Background
While technically not a nuclear receptor, the AhR is mechanistically and functionally similar to members of that super-family, being both a receptor and a ligand-activated transcription factor. More formally, the AhR is a member of the basic helix-loop-helix, Per-Arnt-Sim family of transcription factors. As its name implies, the Aryl Hydrocarbon Receptor (AhR) is a xenobiotic-sensing receptor. It is responsible for the adverse toxicologic effects elicited by various polycyclic aromatic hydrocarbon environmental and industrial pollutants, perhaps the most infamous being 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
The AhR is present in the cytosol where, in the non-active state, it is in a complex with chaperone proteins such as Hsp90. Binding of a polycyclic aromatic hydrocarbon to AhR leads to nuclear translocation and association with its partner protein ARNT. The AhRARNT hetero-dimer binds to specific cognate DNA sequence elements known as dioxin/xenobiotic response elements (DRE/XRE) present in the regulatory region of a variety of target genes. Binding of AhR:ARNT to these elements, and subsequent recruitment of transcription co-activator complexes, induces the transcription of a battery of target genes, including several cytochrome P450 genes. Other target genes of the TCDD/AhR-complex encode inhibitory or stimulatory growth factors that mediate cellular growth and differentiation.
INDIGO’s Zebrafish Aryl Hydrocarbon Receptor (zfAhR) Reporter Cells are constructed in proprietary zebrafish cells. This zebrafish cell line has been modified to contain the luciferase reporter gene functionally linked to tandem DRE/XRE genetic response elements and a minimal promoter sequence. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against the native zebrafish AhR.
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