Zebrafish Peroxisome Proliferator-Activated Receptor Gamma (zPPARγ; nr1c3)
| Product Family | Product Number | Product Description | Technical Manual |
| Z0010 zPPARγ (nr1c3) |
Z00101-32 | Zebrafish PPARγ Reporter Assay System, 3 x 32 assays in 96-well format | Technical Manual |
| Z00101 | Zebrafish PPARγ Reporter Assay System, 1 x 96-well format assays | Technical Manual |
Zebrafish Peroxisome Proliferator-Activated Receptor Gamma Assay Kit 
This Zebrafish Peroxisome Proliferator Activated Receptor Gamma (zPPARγ) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to zPPARγ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
INDIGO’s cell-based reporter assays allow scientists to detect any biological activity that their test samples may exert against a specific receptor present in the cell. They utilize firefly luciferase reporter gene technology which provides superior precision and sensitivity. Since the receptor binding controls the expression of the luciferase reporter gene, luciferase activity in the cells can be correlated directly with the activity of the receptor. The strength of an interaction of a chemical with the target receptor is quantified using a luminometer to measure the level of light emitted.
Fast, reproducible, easy-to-analyze results are only four steps away
Many luciferase reporter assays require the user to grow their own cells and take time to optimize the results. INDIGO’s reporter cells contain the receptor of interest and the luciferase reporter gene. Reporter cells have been optimized to provide extreme sensitivity to quantify even small changes in receptor activity. With INDIGO’s cell-based reporter assays, the process is as easy as Thaw, Feed, Dose, and Read.
Zebrafish PPARγ Reporter Cells
Zebrafish PPARγ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This proprietary cryopreservation process, enables long-term preservation of our unique reporter cells, so we can ship our cryopreserved reporter cells and assay reagents to you via overnight delivery, for your immediate use. Or, you can store the assay kits at -80°C. Once thawed, reporter cells are ready for immediate use so there is no need to take time on intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
Zebrafish PPARγ Luciferase Reporter
INDIGO’s Zebrafish PPARγ assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
PPARg assay kits also available in: Human, Rat/Mouse Shared Ortholog, Cyn Monkey
Zebrafish Peroxisome Proliferator-Activated Receptor Gamma Assay Services
The primary application of INDIGO’s cell-based Zebrafish PPARγ assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of Zebrafish PPARγ. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
Service Assays: Human, Rat/Mouse Shared Ortholog, Cyn Monkey, Zebrafish
Zebrafish Peroxisome Proliferator-Activated Receptor Gamma Assay Background
Peroxisome proliferator-activated receptor gamma (PPAR-gamma or PPARγ), also known as the glitazone receptor, or NR1C3 (nuclear receptor subfamily 1, group C, member 3) is a type II nuclear receptor that in humans is encoded by the PPARG gene. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. PPARγ regulates adipocyte differentiation, fatty acid storage and glucose metabolism. The PPARγ knockout mice fail to generate adipose tissue when fed a high fat diet. Many insulin sensitizing drugs used in the treatment of diabetes target PPARγ as a means to lower serum glucose without increasing pancreatic insulin secretion. Additionally, PPARγ has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.
INDIGO’s Zebrafish Peroxisome Proliferator Activated Receptor Gamma Reporter Assay System utilizes proprietary mammalian cells engineered to provide constitutive, high-level expression of Zebrafish (Danio rerio) PPARγ (nr1c3), a ligand-dependent transcription factor.
INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to an zPPARγ-responsive promoter. Thus, quantifying changes in luciferase expression in the test sample-treated reporter cells provides a sensitive surrogate measure of changes in zPPARγ activity. The principal application of this assay is in the screening of test samples to quantify any functional bioactivity that they may exert against zebrafish PPARγ. In particular, zebrafish reporter assays are frequently used in the monitoring of environmental samples for the presence of biohazardous chemical pollutants, such as endocrine disruptors.
For more information on PPARγ, visit the Nuclear Receptor Resource.
Zebrafish Peroxisome Proliferator-Activated Receptor Gamma Assay Research Areas
Diabetes; Obesity; Cancer; Inflammation; Lipid Metabolism and Energy; Metabolic Disease; NASH/NAFLD; Environmental Toxicology
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