OUR PRODUCT PLATFORM
INDIGO Biosciences’ nuclear receptor assay products are cell-based, reporter assay systems. They feature engineered nuclear receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells typically present greater than 95% cell viability and are ready for immediate use. Test compounds may be screened for agonist or antagonist activities against human nuclear receptors expressed within the cytoplasm and nuclear environments of healthy, dividing mammalian cells.
Our reporter cell systems utilize firefly luciferase reporter gene technology to provide optimal assay sensitivity and dynamic range when quantifying nuclear receptor activity. Following ligand-activation, the nuclear receptor complex acts to induce expression of the luciferase reporter. Upon addition of detection reagent, the intensity of light emission from the luciferase reaction directly correlates to the activation status of the nuclear receptor. All of INDIGO Biosciences’ nuclear receptor reporter assay systems incorporate a detection reagent specially formulated to provide stable light emission over at least 1 hour, thus allowing users to dispense detection reagent into all assay wells prior to commencing activity measurements. This eliminates the need for a luminometer equipped with injectors, allows plates to be processed in batch, and dramatically reduces the start-to-finish read time of assay plates.
PRODUCT FORMATS
INDIGO Biosciences’ nuclear receptor reporter assays are optimally configured for processing in 96- and 384-well format assay plates. Single plate kits (1x 96- and 1x 384-well) are offered as all-inclusive assay systems. Each kit includes nuclear receptor reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual.
Bulk Reagent Packs are offered to accommodate the needs of HTS users. These products are scaled for 10x 96- and 10x 384-well plate assays and provide nuclear receptor reporter cells, optimal culture media, positive-control ligand, luciferase detection reagent, and a detailed Technical Manual and Protocol Quick Guide. Bulk Reagent Packs do NOT include assay plates because HTS facilities typically stock large quantities of such plates.
Please note that, regardless of assay format, Nuclear Receptor Reporter Cells are single-use reagents. Once thawed, Reporter Cells may not be refrozen or reused. Assay kits provide sufficient extra volumes of all reagents to accommodate the needs of performing mechanical transfers and test compound dilutions. However, extra volumes of reporter cells and Luciferase Detection Reagent should be discarded after each assay set-up.
Five basic configurations of our nuclear receptor reporter assay System kits are offered:
- 3x 32 assays in a 96-well format plate.
This all-inclusive kit contains reporter cells and sufficient materials to perform three distinct groups of 32 assays. Each group will comprise 32 nuclear receptor assays, and will utilize four 8-well strips affixed to a 96-well frame (provided). If desired, however, reagents may be combined to perform either 64 or all 96 assay reactions at one time. - 1x 96-well assay.
This all-inclusive kit format contains reporter cells and all additional materials needed and is intended for users wishing to dedicate one entire 96-well plate to performing nuclear receptor assays. - 1x 384-well assay.
This all-inclusive kit format is intended for users wishing to dedicate one entire 384-well plate to performing nuclear receptor assays. - 960 assay Bulk Reagent Pack for (10x) 96-well plates.
This kit format is intended to accommodate HTS applications in which a minimum of ten (10) 96-well plates will be dedicated to a single screening run. Assay plates are NOT supplied with this product. Hence, users must provide white, sterile, cell-culture treated 96-well assay plates. - 3,840 assay Bulk Reagent Pack for (10x) 384-well plates.
This kit format is intended to accommodate HTS applications in which a minimum of ten (10) 384-well plates will be dedicated to a single screening run. Assay plates are NOT supplied with this product. Hence, users must provide white, sterile, cell-culture treated 384-well assay plates.
All Bulk Reagent Packs are manufactured on-demand, and require a maximal lead time of 12 business days for delivery. We welcome inquiries pertaining to the custom manufacture of alternative volume Bulk Reagent Packs. Please let us know how we may better help you achieve your research goals!
All Nuclear Receptor Reporter Assay System products are shipped on dry ice for overnight delivery anywhere within the United States.
HOW TO USE OUR KITS: A QUICK GUIDE VIDEO
Perform nuclear receptor assays with easy-to-use kits from INDIGO Biosciences. This short video provides a brief overview of what to expect when working with our kits, including unpacking and storing kit contents, setting up and performing the assays, and reviewing data and results.
*Please note, this video is for INDIGO’s non-pre-incubation assays. INDIGO’s pre-incubation assays involve minor but crucial additional Day 1 steps and different dispensed volumes. Always refer to your Technical Manual (included in all kit shipments) for the most up-to-date information and proper technique. Find out which kits require pre-incubation steps here.