Luciferase Reporter Technology for Superior Sensitivity
Say goodbye to weeks of cell culture and optimization and achieve superior sensitivity with INDIGO’s Luciferase Reporter Assays, designed to generate quality data within 24 hours.
Superior Sensitivity and Selectivity
INDIGO’s robust Luciferase Reporter Assays are optimized to provide extreme sensitivity with minimal risk of background noise.
High Reproducibility and Repeatability
Generate highly reproducible, repeatable data and move your screening project forward with confidence.
Reliable Quantitative Measurements
Easily measure your signal and accurately quantify changes in activity of your receptor of interest.
Your Results, Only 24 Hours Away
Make decisions faster by bringing our luciferase technology into your workflow. Through four simple steps, you can get the insights you need within 24 hours! Our Luciferase Receptor Reporter Assay Kits contain reporter cells transfected with the luciferase reporter gene and your receptor of interest. Try the INDIGO way, as easy as Thaw, Feed, Dose, and Read!
Our cryopreserved reporter cells and assay reagents are ready to ship via overnight delivery. Once thawed, our cells are ready for immediate use with no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
Our simple protocol gets you results in as little as 24 hours!
See Our Ready-to-use Luciferase Receptor Assay Kits in Action
Perform Luciferase Reporter Assays quickly and accurately with INDIGO Biosciences’ easy-to-use Kits. This video provides an overview of our 96-well format kits, including unpacking and storing contents, setting up and performing the assay, as well as data analysis.
Three Assay Formats to Meet Your Needs
The Secret to INDIGO’s Luciferase Receptor Assay Kits’ Performance
INDIGO’s Highly Selective and Sensitive Luciferase Reporter Technology
INDIGO’s assays use Luciferase Reporter Technology, a light emission method designed for unparalleled sensitivity, selectivity, and dynamic range.
Our Luciferase Reporter Cells incorporate cDNA encoding luciferase, a 62 kD protein from the North American firefly (Photinus pyralis). Luciferase catalyzes the oxidation of D-luciferin, resulting in light production, quantifiable using a luminometer and reported in Relative Light Units (RLU’s).
In INDIGO’s Assays, luciferase transcription is triggered by activating a specific receptor, lowering off-target risks and unlocking superior selectivity. A test compound binds to the receptor if biologically active, inducing quantifiable luciferase expression.
With our highly sensitive, selective, and cell-culture-free Luciferase Reporter Assays, you can confidently screen test compounds for agonist or antagonist activities in record time.
Long-Term Cell Preservation with INDIGO’s Proprietary CryoMite™ Process
All INDIGO Biosciences’ Luciferase Reporter Assays feature engineered receptor-specific reporter cells prepared using the proprietary CryoMite™ process. Our cryopreservation process enables the long-term preservation of our unique reporter cells.
Get your cryopreserved Luciferase Reporter Assay Kits overnight and start generating reliable data in record time. Last minute change? Just store your kits at -80°C for easy access.
Transiently Transfected Cells for More Reliable and Accurate Data
INDIGO Biosciences’ reporter cells use transient transfection to ensure reliable and accurate results. Our cells are optimized to provide the best experimental conditions with the reagents provided in our kits, allowing for easy experiment standardization and reproducibility.
Additionally, there is no need for costly licensing agreements when purchasing and using stable cell lines.