Toxicology Assay Kits
Rapidly assess therapeutic candidates’ potential for drug-drug interactions and accurately predict xenobiotic-induced liver toxicity. INDIGO’s toxicology assay kits provide rapid, reliable results for:
Showing 1–10 of 19 results
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Cyn Monkey PXR Reporter Assay Kit
Species
Cyn Monkey
Target Type
Nuclear Hormone Receptor
Nuclear Receptor Orthologs
Associated Disease
Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
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Dog PXR Reporter Assay Kit
Species
Dog
Target Type
Nuclear Hormone Receptor
Nuclear Receptor Orthologs
Associated Disease
Cancer, Cardiovascular, Kidney Disease, NASH/NAFLD, Wound Healing
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Expression Profiling of Clinically Relevant CYPs Assay Kit
Species
Human
This Expression Profiling of Clinically Relevant CYPs assay kit contains optimized reagents for the culturing and treatment of upcyte® hepatocytes to assess drug-induced changes in CYP3A4, CYP1A1, CYP2B6, CYP2C8, CYP2C9, CYP2C19, and CYP2E1.
The kit provides two aliquots of upcyte® hepatocytes, two cell culture-ready assay plates, optimized Cell Culture Medium for use in all steps of the assay procedure, and three reference compounds (rifampicin, β-naphthoflavone, and CDCA) that activate one or more of the primary xenobiotic-sensing receptors: PXR, CAR, AhR, and FXR. Upon activation, these nuclear receptors modulate the expression of the CYP genes. Also included are seven sets of validated qPCR primers for quantifying drug-induced changes in the expression of CYP3A4, CYP1A1, CYP2B6, CYP2C8, CYP2C9, CYP2C19, and CYP2E1, as well as primers for ACTB.
Please note: This kit does not include reagents or protocols for cell lysis, RNA isolation, cDNA preparation, or qPCR assays.
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Human AhR Reporter Assay Kit
Species
Human
Target Type
Nuclear Hormone Receptor
Associated Disease
Cancer, Cardiovascular, Dermatitis, Kidney Disease, NASH/NAFLD, Reproduction, Wound Healing
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Human AP-1 Reporter Assay Kit
Species
Human
Target Type
Transcription Factor
Associated Disease
Cancer
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Human CAR-2 Reporter Assay Kit
Species
Human
Target Type
Nuclear Hormone Receptor
Associated Disease
NASH/NAFLD, Wound Healing
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Human CAR-3 Reporter Assay Kit
Species
Human
Target Type
Nuclear Hormone Receptor
Associated Disease
NASH/NAFLD, Wound Healing
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Human NF-kB Reporter Assay Kit
Species
Human
Target Type
Transcription Factor
Associated Disease
Autoimmune, Cancer, Cardiovascular, Dermatitis, Kidney Disease, Neurodegenerative
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Human Nrf2 Reporter Assay Kit
Species
Human
Target Type
Nuclear Hormone Receptor
Associated Disease
Cancer, Cardiovascular, NASH/NAFLD, Neurodegenerative, Obesity, Reproduction
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Human P-Glycoprotein / MDR1 Drug Interaction Assay
Species
Human
The primary application of INDIGO Biosciences MDR1 drug interaction assay kit is to rapidly assess drug candidates as either inhibitors, substrates, or non-substrates of P-glycoprotein. This is accomplished by co-treating the cells with varying concentrations of the test drug and a fixed concentration of daunorubicin, a well-characterized fluorescent substrate for P-glycoprotein mediated transport. HCT-Pgp cells are a proprietary cell line that have been extensively selected for high-level expression of native P-glycoprotein.
The kit contains two aliquots of HCT-Pgp cells and two black, sterile, collagen-coated 96-well assay plates. In addition to HCT-Pgp cells and assay plates, this kit includes Cell Recovery Medium (CRM-p) used to perform a rapid thaw of the -80°C HCT-Pgp cells, Compound Screening Medium supplemented with daunorubicin (CSM+DR) used to prepare drug treatment media, Wash Buffer, two aliquots of Lysis Reagent, and the potent reference inhibitor Tariquidar.