Environmental Testing Solutions from INDIGO
INDIGO’s effects-based bioassay solutions compliment traditional analytical chemistry approaches for the examination of water quality and can help protect human and environmental health.
Contaminant Detection and Quantification
Human-impacted surface, ground and drinking water can contain a complex mixture of micropollutants, such as pharmaceuticals, pesticides, and industrial compounds. These potentially harmful chemicals are often at low concentrations, and targeted chemical analysis cannot detect all the hazards present. Suspected chemical screening and non-target analysis can identify a larger number of compounds but cannot provide any information about the potential toxic effects of the micropollutant mixture. Effect-based biomonitoring can be applied in parallel to chemical analysis to detect the effect of all known and unknown chemicals in an environmental sample. Bioassays can account for mixture effects such as additivity, synergy, antagonism and are risk-scaled as more potent chemicals have a greater effect in the assay. Consequently, effect-based methods have been recommended to complement chemical analysis in water quality monitoring.
INDIGO has an extensive list of cellular bioassays that are predictive of cellular toxicity pathways including endocrine disruption, altered xenobiotic metabolism and adaptive stress responses.
INDIGO's assays can detect the cumulative effect of bioactive compounds in extracted environmental matrices and provide important biological context. They are:
- All-Inclusive
- Sensitive
- Cost Effective
- Reproducible
- and Easy to Perform
Learn more about INDIGO Biosciences’ Assay Kit Platforms & Formats
INDIGO has an extensive list of bioassay kits for receptors important in Environmental Monitoring for contaminant detection and quantification in samples which include:
- AR (NR3C4)
- AhR
- ERα (NR3A1)
- ERβ (NR3A2)
- ERRα (NR3B1)
- ERRγ (NR3B3)
- GR (NR3C1)
- Nrf2
- PGR (NR3C3)
- PPARγ (NR1C3)
- TRα (NR1A1)
- TRβ (NR1A2)
Our receptor specific assays are cell-based reporter assay systems. They feature engineered receptor-specific reporter cells prepared using our unique CryoMite™ process. Once thawed, reporter cells are ready for immediate use. Test compounds can be screened for agonist or antagonist activities against receptors.
INDIGO Biosciences works closely with clients to provide the appropriate reporter specific assays for their water testing needs. Our technology generates clear single receptor or full-panel screening results. Employing a luminescence-based method and our proprietary CryoMite™ preservation process, INDIGO kits provide reproducible results lot-to-lot.
Learn more about INDIGO Biosciences’ Assay Kit Platforms & Formats