All INDIGO Assays
INDIGO Biosciences’ cell-based, luciferase reporter assay kits feature receptor-specific reporter cells prepared using our unique CryoMite™ process which enables long-term preservation of our unique reporter cells, so we can ship our cryopreserved reporter cells and assay reagents for your immediate use. INDIGO’s assay kits are engineered to provide extreme assay sensitivity to quantify even small changes in receptor activity.
Many labs are interested in cross-species comparisons, especially contrasting human nuclear receptor activity to common laboratory animals. Determining a drug candidate’s cross-activity with human xenobiotic-sensing receptors provides important early indications of that drug’s potential for downstream drug-drug interactions. With animal studies required by the FDA, selecting the animal model that provides the most representative human-surrogate is critical to assessing a potential drug’s likelihood of unwanted effects. Cell-based assay models are crucial to help make this determination prior to entering ADMET studies.
With more than 30 ortholog assays - including rat, mouse, dog, monkey, and zebrafish - available as kits and/or services, and others available for custom development, INDIGO helps researchers screen the right animal, before trial.
| Product Family | Cyn Monkey | Dog | Mouse | Rabbit | Rat | Zebrafish |
|---|---|---|---|---|---|---|
| Androgen Receptor | rAR | zAR | ||||
| Aryl Hydrocarbon Receptor | mAhR | rAhR | zAhR | |||
| Constitutive Androstane Receptor | mCAR | |||||
| Estrogen Receptor Alpha | rERα | zERα | ||||
| Estrogen Receptor Beta | rERβ | |||||
| Farnesoid X Receptor | cFXR | dFXR | mFXR | rFXR | ||
| Glucocortioid Receptor | mGR | rGR | zGR | |||
| Liver X Receptor Alpha | dLXRα | mLXRα | rLXRα | |||
| Liver X Receptor Beta | dLXRβ | mLXRβ | rLXRβ | |||
| Peroxisome Proliferator-Activated Receptor Alpha | cPPARα | dPPARα | mPPARα | rPPARα | ||
| Peroxisome Proliferator-Activated Receptor Beta/Delta | cPPARβ/δ | dPPARβ/δ | mPPARβ/δ | rPPARβ/δ | ||
| Peroxisome Proliferator-Activated Receptor Gamma | cPPARγ | m/rPPARγ | m/rPPARγ | zPPARγ | ||
| Pregnane X Receptor | cPXR | dPXR | mPXR | rPXR | ||
| Progesterone Receptor | cPGR | rbPGR | rPGR | |||
| Retinoic Acid Receptor Alpha | zRARαa | |||||
| RAR-related Orphan Receptor Gamma | mRORγ | rRORγ | ||||
| Thyroid Hormone Receptor Beta | zTRβ |
INDIGO’s nuclear receptor reporter assay panels contain all materials needed to perform either 32 or 48 assays for each receptor included. PPAR, RAR, and RXR Panels are available in a 3x32-well format; ER, LXR, and TR Panels are in a 2x48-well format, all designed to fully utilize a single 96-well plate. All reagents are supplied with sufficient extra volume to accommodate the needs of performing separate groups of assays. When screening a smaller number of compounds against all variants of a receptor, INDIGO’s Assay Panel kits allow researchers to save time, cost, and risk by screening against multiple receptors in one easy-to-use kit.
INDIGO’s Bioassays for Environmental Sample Monitoring were specifically developed for use in environmental testing applications such as screening for endocrine disruptors and other contaminants of emerging concern. The data provided by the assays allow users to assess a sample’s bioactivity relative to a standard reference compound and compare it to an established concentration of concern.
AhR Bioassay for Environmental Sample Monitoring
AR Bioassay for Environmental Sample Monitoring