Product Search Reset all × in vitro Hepatotox × search: fxr × 1 product found × Target Receptor Expression Profiling of Clinically Relevant CYPs (1) Species Human (1) Assay Mode— in vitro Hepatotox Agonist (6) Antagonist (6) Gene Expression (1) in vitro Hepatotox (1) Show (1) Cancel Reset all × in vitro Hepatotox × search: fxr × Showing the single result Default sorting Sort by popularity Sort by latest Sort by price: low to high Sort by price: high to low Expression Profiling of Clinically Relevant CYPs Assay KitSpeciesHumanThis Expression Profiling of Clinically Relevant CYPs assay kit contains optimized reagents for the culturing and treatment of upcyte® hepatocytes to assess drug-induced changes in CYP3A4, CYP1A1, CYP2B6, CYP2C8, CYP2C9, CYP2C19, and CYP2E1. The kit provides two aliquots of upcyte® hepatocytes, two cell culture-ready assay plates, optimized Cell Culture Medium for use in all steps of the assay procedure, and three reference compounds (rifampicin, β-naphthoflavone, and CDCA) that activate one or more of the primary xenobiotic-sensing receptors: PXR, CAR, AhR, and FXR. Upon activation, these nuclear receptors modulate the expression of the CYP genes. Also included are seven sets of validated qPCR primers for quantifying drug-induced changes in the expression of CYP3A4, CYP1A1, CYP2B6, CYP2C8, CYP2C9, CYP2C19, and CYP2E1, as well as primers for ACTB. Please note: This kit does not include reagents or protocols for cell lysis, RNA isolation, cDNA preparation, or qPCR assays.