View the slide viewer

Have questions about this assay kit?

Human ADRA1D Reporter Assay Kit

1 x-384 well format assays
1 x-96 well format assays
1 x-384 well format assays
1 x-96 well format assays

Product Description and Product Data

This is an all-inclusive cell-based luciferase reporter assay kit targeting the the Human Adrenoreceptor Alpha 1D (ADRA1D). INDIGO’s ADRA1D reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ADRA1D. In addition to ADRA1D Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against ADRA1D. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.


  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeGPCR
Receptor FormHybrid
Assay ModeAgonist, Antagonist
Kit Components
  • Human ADRA1D Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • L-Phenylephrine, (ref. agonist; in DMSO)
  • Detection Substrate
  • Detection Buffer
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Shipping RequirementsDry Ice
Storage temperature-80C


Activation of ADRA1D. Activation assays were performed using the reference compounds L-phenylephrine (provided), Cirozoline*HCl, Epinephrine, Norepinephrine, A-61603, and Clonidine.
Inhibition of ADRA1D. ADRA1D reporter cells were co-treated with an EC80 concentration of the reference activator L-phenylephrine and varying concentrations of the general alpha adrenergic receptor inhibitors, BMY7378, WB4104, Doxazosin mesylate, Carvedilol, HEAT Hydrochloride, Silodosin and Terazosin. INDIGO’s Live Cell Multiplex (LCM) Assay confirmed that no treatment concentrations were cytotoxic (data not shown).

Target Background

The adrenoreceptors (a.k.a. adrenergic receptors) mediate the action of the sympathetic nervous system and are activated in response to “fight-or-flight” signals. They are divided into three types, adrenoreceptor α1-, α2-, and β. Each type is further composed of three subtypes resulting in 9 different types (α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, and β3).

Adrenoreceptors belong to the G-Protein-coupled receptor (GPCR) family. They all display the characteristic seven transmembrane helices, the extracellular loops which contribute to ligand binding, and the intracellular carboxy tail that associates with trimeric G proteins. All nine types of adrenoreceptors are activated by the same endogenous catecholamines (epinephrine and norepinephrine); however, the specificity of their responses depends on the G-proteins and effectors systems they associate with in a tissue and time specific manner.

Adrenoreceptor alpha 1D (ADRA1D) signals through the Gαq/11 family of G proteins. Upon binding to a catecholamine, ADRA1D undergoes a conformational change that triggers the activation of Gαq/11 proteins via an exchange of GDP with GTP, followed by the activation of phospholipase C, the release of inositol triphosphate (IP3) which binds to its receptors on the endoplasmic reticulum and triggers the release of calcium and activation of the protein kinase C.

ADRA1D is abundantly expressed in the brain3. ADRA1D is highly distributed in dentate nucleus neurons of the cerebellum. Major roles of ADRA1D include involvement in cardiovascular, urinary and central nervous system functions4. Although ADRA1D is closely related to ADRA1A and ADRA1B the precise physiological roles of ADRA1D have yet to be firmly established.

Also available as a service

Adrenoreceptor Alpha 1D (ADRA1D)

Have questions about this assay kit?