Transcription Factor Assay Kits: Unveil the Possibilities

Unveil the power of our cell-based luciferase reporter Transcription Factor Assay Kits for your research.

Enable in-depth investigations into cellular processes and signaling pathways

Gain insights into gene regulation mechanisms by measuring transcription factor activity in live cells


Transcription Factors: Regulating Gene Expression and Cellular Responses

Transcription factors are important proteins that play a crucial role in regulating gene expression by binding to specific DNA sequences. These receptors are often found within the cell's nucleus and act as sensors, detecting signals from the external environment or cellular processes.

Upon signal activation, transcription factors undergo conformational change, bind to DNA, then directly influence gene transcription. By binding to DNA sequences known as response elements, transcription factors can either activate or repress the expression of target genes, and thereby modulate various cellular processes and responses.

Transcription factors are involved in a wide range of biological functions, such as embryonic development, immune response, and hormone signaling. Their ability to integrate external signals and initiate gene expression programs makes them key regulators of cellular behavior and adaptation.

Understanding the mechanisms and functions of transcription factors provides insight into how cells perceive and respond to their environment, thus highlighting their significance in various physiological and pathological contexts.

Frequently Asked Questions

INDIGO’s assays are not binding assays. They are cell-based trans-activation assays, and the principal application is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that the compounds may exert against the nuclear receptors. INDIGO reporter systems utilize firefly luciferase reporter gene technology, and while there is a binding taking place, our assays do not measure it. Instead, the luciferase light response is measured which correlates to the activation status of the receptor (either activation or inhibition). Quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in receptor activity. It will tell the scientist the significance (strength) of the interaction by the level of light emitted. In addition, cell-based assays are more sensitive and able to detect smaller levels of activation.

INDIGO’s nuclear receptor assays utilize proprietary human and non-human mammalian cells engineered to provide constitutive, high-level expression of the designated receptor. Specific cell type information for each assay is proprietary and available only through consultation with INDIGO’s technical team following a screening service or assay kit purchase.

Reporter cells included in INIDIGO’s steroid hormone nuclear assay kits (ERα, ERβ, AR, PGR, MR, GR) express the native, full-length receptors. INDIGO’s other nuclear receptor assays, however, include reporter cells that express hybrid nuclear receptors. In these cases, the respective receptor’s native N-terminal sequence comprising the DNA Binding Domain (DBD) has been replaced with sequence encoding the yeast Gal4-DBD. All other native NR functional/structural domains (ligand binding domain, hinge region, and various activation domains) are present in these hybrid receptors. These reporter cells also contain the firefly luciferase reporter gene functionally linked to the upstream genetic response element for Gal4. Consequently, once a bioactive compound associates with the ligand binding domain of the hybrid receptor, only the luciferase reporter gene is induced. Ligand-activation of the hybrid receptor will not induce collateral expression of target genes that are otherwise regulated by the native nuclear receptor.

View our list of reference compounds. We do utilize commercially available reference agonists for our assays, and this reference agonist is included in each assay kit. We do use reference antagonists where a reference is known and commercially available. Some of our antagonist assays do not have reference antagonists available.

Each kit includes luciferase reporter cells, an assay plate, all required reagents (optimized culture media, positive-control agonist, and detection reagent), and a detailed Technical Manual. A luminometer is required for Relative Light Unit (RLU) measurements.

INDIGO’s kits are intended for research purposes only, and not for diagnostic or therapeutic use in humans.

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