Custom Assay Development Services and Solutions

Discovery and development research depends on reliable, high-performing assays designed to meet your needs. If your R&D requires assays beyond INDIGO’s existing offerings, our experienced team will assist in your research on nuclear receptors, cell-based reporter assays, gene expression, or toxicology screening. We can help create high-throughput NR reporter gene assays, develop ortholog assays, or a variety of other services. We supplement the world’s largest portfolio of nuclear receptor assay kits and services and in vitro toxicology solutions with greater results readability, reproducibility, and faster turnaround times. Our custom assay kit solutions, plus supportive team and reliable science and platforms aim to reduce time, cost, and risk associated with the discovery process. Contact us today and Request a Quote to discuss your research needs.

Examples of Custom Assay Development Services Available

Custom Nuclear Receptor Assay: Interested in a human nuclear receptor that is not in our current portfolio? We can clone and optimize an assay for services use in our laboratories or as assay kits to be utilized in yours.

Ortholog Nuclear Receptor Assay: Many labs are interested in cross-species comparisons, especially contrasting human nuclear receptor activity to common laboratory animals such as rat, mouse, dog, and non-human primate. While INDIGO currently offers numerous NR assays in human, rat, and mouse, along with several in dog and non-human primate, though if you need an assay for a species not currently available, our services lab can clone and optimize an assay for your research.

Selective Receptor Modulation (SRM) Assays: The concept that ligands for members of the NR superfamily should be classified as either “agonists” or as “antagonists” is often an oversimplification. For example, tamoxifen acts in vivo as an estrogen receptor (ER) antagonist in the breast but as an ER agonist in bone and uterus. The reason for these opposite actions is provided by numerous NR-associated proteins such as corepressors / coactivators that interact with a particular ligand receptor complex. Thus a new concept of selective receptor modulators (SRMs) has emerged to account for the different contextual actions of NR ligands, depending on the cellular milieu in which this chemical acts. INDIGO has at its disposal a variety of coactivator and corepressor constructs, as well as LXXLL containing peptides for examination of protein-protein interaction in the mammalian to-hybrid assay.

Nuclear Receptor Assay in a Specific Cell Line: INDIGO’s team has optimized its reporter assays in particular cell line, which vary depending on the receptor. If this cell line is not where you need the information, let INDIGO’s staff know and we can develop and optimize the appropriate conditions.

Nuclear Receptor Assay with a Specific Reporter: Similar to using a specific cell line, if our optimized reporter gene is not what you need, INDIGO will incorporate a different reporter gene in the customized assay.

Please note: Custom assays are developed under a non-exclusive arrangement. Although the constructs developed for the client may be sent for their own use, INDIGO retains the right to add the newly developed assay as part of our services and/or kit portfolio.