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In Vitro Hepatotoxicity and DILI Screening
Evaluate liver safety liability earlier using hepatocyte-based in vitro models and metabolism-enabled screening assays.
Drug-induced liver injury remains a major cause of compound attrition and development risk. INDIGO’s hepatotoxicity screening services help discovery and safety teams identify concentration-dependent and metabolism-dependent liver toxicities, rank compounds, and make better-informed decisions before moving into more complex or costly studies.
Using upcyte® human hepatocytes, and other methods, INDIGO provides practical, reproducible liver safety data designed to support lead optimization, analog comparison, early DILI risk assessment, and mechanistic follow-up.
Earlier Insight into Liver Liability
Liver toxicity can emerge from direct cellular injury, metabolism-related effects, mitochondrial stress, oxidative stress, transporter interactions, or broader pathway disruption. Early in vitro hepatotoxicity screening helps identify compounds that may require optimization, deprioritization, or additional mechanistic evaluation.
INDIGO’s hepatotoxicity services are designed to help you move beyond simple viability screening. Our scientists can help select appropriate study conditions, concentration ranges, exposure windows, and follow-up assays to generate data that are relevant to your stage of development and research question.
Use hepatotoxicity screening when you need to:
- Prioritize compounds or analogs for liver safety liability
- Generate concentration-response data for early DILI risk assessment
- Compare lead candidates before candidate selection
- Investigate an observed cytotoxicity or liver-related signal
- Evaluate parent compound and metabolite-related effects
- Support a broader in vitro toxicology or ADME/Tox strategy
- Determine whether additional mechanistic follow-up is needed
Clear outputs for compound ranking and decision-making
Depending on study design, INDIGO hepatotoxicity data packages may include:
- Cell viability or toxicity readouts
- Concentration-response curves
- EC50 and IC50 values when applicable
- Assay controls and quality metrics
- Raw data
- GraphPad Prism files when applicable
- PDF study report
- Scientist interpretation and recommended next steps
Human-relevant upcyte® hepatocyte-based screening
INDIGO’s hepatotoxicity assays use the upcyte® human hepatocyte model as the primary platform for practical early liver safety screening. upcyte® hepatocytes provide a fit-for-purpose balance of human liver relevance, reproducibility, and screening efficiency, allowing teams to assess multiple compounds or concentration ranges in a consistent, scalable hepatocyte-based system.
By evaluating concentration-dependent effects across compounds or conditions, INDIGO helps generate data that can support compound ranking, internal decision-making, and follow-up study design.
For clients with species comparison requirements, such as bridging human and rodent or non-human primate metabolism data, INDIGO welcomes the opportunity to discuss custom study designs using animal model hepatocytes.
Why Choose INDIGO’s Hepatotoxicity Services?
upcyte® human hepatocyte model, S9 fractions, with custom species comparison available
Evaluate liver safety liability using the upcyte® human hepatocyte model, designed to provide more liver-relevant insight than generic immortalized cell viability assays alone, with additional animal model hepatocyte and S9 fraction options available to support species comparison and metabolism-related study designs.
Concentration-response data
Generate quantitative data to support compound prioritization, potency estimation, and decision-making.
Integration with broader mechanistic safety services
Move from initial liver toxicity screening into metabolism-dependent testing, CYP450 assays, receptor profiling, or gene expression follow-up with one scientific team.
Scientist-led study design
Work with INDIGO scientists to align assay conditions with your compound class, stage of development, and internal decision needs.
Get Reliable Data & Clear Reports from Our Expert Scientists
Get Reliable Data & Clear Reports from Our Expert Scientists
Full study report with IC₅₀ values and viability curves
Raw data and processed results in publication-ready format
Optional consultation to interpret results or plan follow-up testing
