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Human GPR39 Reporter Assay Kit

SIZE SKU PRICE
1 x-96 well format assays—
SIZE SKU
1 x-96 well format assays
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Product Description and Product Data

This is an all-inclusive cell-based luciferase reporter assay kit targeting the the Human G-Protein-Coupled Receptor 39 Reporter Assay System (GPR39). INDIGO’s GPR39 reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the GPR39. In addition to GPR39 Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against GPR39. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.

Features

  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeGPCR
SpeciesHuman
Receptor FormHybrid
Assay ModeActivation, Inhibition
Kit Components
  • GPR39 Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • GPR39-C3, 200 μM (in DMSO)
  • Detection Substrate
  • Detection Buffer
  • 96-well, collagen-coated assay plate (white, sterile, cell-culture ready)
Shelf Life6 months
Shipping RequirementsDry Ice
Storage temperature-80C

Data

Activation of GPR39. Assay was performed using the reference compound GPR-39-C3 (Cayman Chemical, Ann Arbor, MI). Luminescence was quantified and values of average (n=6) relative light units (RLU), corresponding standard deviation (SD), Fold-Activation, and Z’ values were calculated. GraphPad Prism software was used to plot data using the least-squares method of non-linear regression for Fold- Activation vs. Log10 [GPR39-C3, nM], and to determine the EC50 value.

Target Background

This assay utilizes proprietary human cells that provide constitutive expression of the Human G-Protein-Coupled Receptor 39 (GPR39).

G-protein-coupled receptors (GPCRs) play a pivotal role in mediating responses to various hormones, neurotransmitters, and external stimuli, making them essential therapeutic targets for multiple conditions. GPR39, an orphan GPCR, is widely expressed in diverse tissues such as the pancreas, gastrointestinal tract, liver, kidneys, skin, adipose tissue, and brain.

GPR39 is associated with neurodegenerative diseases such as Alzheimer’s, dementia, epilepsy and depression. In addition, recent evidence has highlighted the role of GPR39 in metabolic and endocrine diseases such as diabetes and obesity. Consequently, GPR39 commands considerable interest in therapeutics development.

Citations

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G-Protein-Coupled Receptor 39 Reporter Assay System (GPR39)

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