View the slide viewer

Have questions about this assay kit?

Glucocorticoid Receptor Bioassay for Environmental Sample Monitoring

1 x-96 well format assays
1 x-96 well format assays

Product Description and Product Data

This Glucocorticoid Receptor bioassay kit for environmental sample monitoring is an all-inclusive luciferase reporter assay system that includes, in addition to GR Reporter Cells, two optimized media for use during cell culture and in diluting the user’s test samples, Luciferase Detection Reagent, a cell culture-ready assay plate. and a detailed protocol.


  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor
Receptor FormNative
Assay ModeAgonist, Antagonist
Kit Components
  • GR Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • Dexamethasone, (ref. agonist; in DMSO)
  • Luciferase Detection Reagent
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Orthologs AvailableYes
Shipping RequirementsDry Ice
Storage temperature-80C


The reference agonist Dexamethasone (Dex; provided) is used to generate a standard curve following the dilution scheme presented in APPENDIX 1 of the technical manual. Average relative light units (RLU) and corresponding values of Percent Coefficient of Variation (%CV) were determined for each treatment concentration (n = 3). Values of Percent Activation (relative to the EC100 concentration of Dex) were calculated for each treatment concentration of Dex, as described in Step 19e. GraphPad Prism software was used to plot Percent Relative Activation vs. Log10[Dex, pM] using the 4-parameter, least squares method of non-linear regression; the bottom plateau of the sigmoidal curve is constrained to ‘0’.

Target Background

The glucocorticoid receptor is the receptor that cortisol and other glucocorticoids bind to. The GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response.

INDIGO’s Glucocorticoid Receptor Assay System utilize proprietary mammalian reporter cells engineered to provide constitutive high-level expression of full-length, unmodified human NR3C1 protein, commonly referred to as GR.

INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in GR activity. Luciferase gene expression occurs after ligand-bound GR undergoes nuclear translocation, DNA binding, recruitment, and assembly of the co-activators and accessory factors required to forma functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human androgen receptor.

Also available as a service

Glucocorticoid Receptor (GR, NR3C1)

Have questions about this assay kit?