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Estrogen Receptor Bioassay for Environmental Sample Monitoring

1 x-96 well format assays
1 x-96 well format assays

Product Description and Product Data

This Estrogen Receptor bioassay kit for environmental sample monitoring is an all-inclusive luciferase reporter assay system that includes, in addition to ER Reporter Cells, two optimized media for use during cell culture and in diluting the user’s test samples, Luciferase Detection Reagent, a cell culture-ready assay plate. and a detailed protocol.


  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor
Receptor FormNative
Assay ModeAgonist, Antagonist
Kit Components
  • ER Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM) 17b-Estradiol (ref. agonist; in DMSO)
  • Luciferase Detection Reagent
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Orthologs AvailableYes
Shipping RequirementsDry Ice
Storage temperature-80C


The reference agonist 17β-estradiol (E2; provided) is used to generate a standard curve following the dilution scheme presented in APPENDIX 1. Average relative light units (RLU) and corresponding values of Percent Coefficient of Variation (%CV) were determined for each treatment concentration (n = 3). Values of Percent Activation (relative to the EC100 concentration of E2) were calculated for each treatment concentration of E2, as described in Step 19e. GraphPad Prism software was used to plot Percent Relative Activation vs. Log10[E2, pM] using the 4-parameter, least squares method of non-linear regression; the bottom plateau of the sigmoidal curve is constrained to ‘0’.

Target Background

Estrogen receptor alpha (ER-alpha), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is a nuclear receptor which is activated by the sex hormone estrogen. ER-alpha is encoded by the human gene ESR1 (Estrogen Receptor 1).The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.

Estrogen receptor beta (ER-beta), also known as NR3A2 (nuclear receptor subfamily 3, group A, member 2), is a nuclear receptor which is activated by the sex hormone estrogen. ER-beta is encoded by the human gene ESR2 (Estrogen Receptor 2). This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. ER-β is expressed by many tissues including blood monocytes and tissue macrophages, colonic and pulmonary epithelial cells and in prostatic epithelium and in malignant counterparts of these tissues.ER-β may have anti-proliferative effects and therefore oppose the actions of ER-α in reproductive tissue.[4] ER-β may also have an important role in adaptive function of the lung during pregnancy.

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