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Mineralocorticoid Receptor Bioassay for Environmental Sample Monitoring

1 x-96 well format assays
1 x-96 well format assays

Product Description and Product Data

This Mineralocorticoid Receptor bioassay kit for environmental sample monitoring is an all-inclusive luciferase reporter assay system that includes, in addition to MR Reporter Cells, two optimized media for use during cell culture and in diluting the user’s test samples, Luciferase Detection Reagent, a cell culture-ready assay plate. and a detailed protocol.


  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor
Receptor FormNative
Assay ModeAgonist, Antagonist
Kit Components
  • MR Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • Aldosterone, (ref. agonist; in DMSO)
  • Luciferase Detection Reagent
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Orthologs AvailableNo
Shipping RequirementsDry Ice
Storage temperature-80C


Generating a Standard Curve of MR activity. The reference agonist Aldosterone (provided) is used to generate a standard curve following the dilution scheme presented in APPENDIX 1 in the technical manual. Average relative light units (RLU) and corresponding values of Percent Coefficient of Variation (%CV) were determined for each treatment concentration (n = 3). Values of Percent Activation (relative to the EC100 concentration of Aldosterone) were calculated for each treatment concentration of Aldo, as described in Step 19e. GraphPad Prism software was used to plot Percent Relative Activation vs. Log10[Aldo, pM] using the 4-parameter, least squares method of non-linear regression; the bottom plateau of the sigmoidal curve is constrained to ‘0’.

Target Background

The mineralocorticoid receptor (MR), also called aldosterone receptor, is a receptor with high affinity for mineralocorticoids. It belongs to the steroid hormone receptor family where the ligand diffuses into cells, interacts with the receptor, and results in a signal transduction affecting specific gene expression in the nucleus. The gene for the NR3C2 (located on chromosome 4q31.1-31.2) encodes for the 107 kDa MR protein.

MR is expressed in many tissues, such as the kidney, colon, heart, central nervous system (hippocampus), brown adipose tissue, and sweat glands. In epithelial tissues, its activation leads to the expression of proteins regulating ionic and water transports (mainly the epithelial sodium channel or ENaC, Na+/K+ pump, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsorption of sodium, and as a consequence an increase in extracellular volume, increase in blood pressure, and an excretion of potassium to maintain a normal salt concentration in the body.

The receptor is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, like cortisol and cortison. It also responds to some progestins. Spironolactone and eplerenone are mineralocorticoid receptor antagonists. Activation of the mineralocorticoid receptor, upon the binding of its ligand aldosterone, results in its translocation to the cell nucleus, homodimerization and binding to hormone response elements present in the promoter of some genes. This results in the complex recruitment of the transcriptional machinery and the transcription into mRNA of the DNA sequence of the activated genes.

INDIGO’s Mineralocorticoid Receptor Reporter Assay Systems utilize proprietary mammalian cells engineered to express human NR3C2 protein, commonly referred to as MR.

The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human mineralocorticoid receptor.

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Mineralocorticoid Receptor

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