Estrogen Receptors Panel (ERα, ERβ)
This PANEL of Estrogen Receptors Reporter Assays utilizes non-human mammalian cells engineered to express distinct human ERα (NR3A1) and ERβ (NR3A2) proteins.
Estrogen receptor alpha (ER-alpha), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is a nuclear receptor which is activated by the sex hormone estrogen. ER-alpha is encoded by the human gene ESR1 (EStrogen Receptor 1).The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.
Estrogen receptor beta (ER-beta), also known as NR3A2 (nuclear receptor subfamily 3, group A, member 2), is a nuclear receptor which is activated by the sex hormone estrogen. ER-beta is encoded by the human gene ESR2 (EStrogen Receptor 2). This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. ER-β is expressed by many tissues including blood monocytes and tissue macrophages, colonic and pulmonary epithelial cells and in prostatic epithelium and in malignant counterparts of these tissues.ER-β may have anti-proliferative effects and therefore oppose the actions of ER-α in reproductive tissue.[4] ER-β may also have an important role in adpative function of the lung during pregnancy.
This PANEL contains sufficient materials to perform 48 ERα assays and 48 ERβ assays in 96-well plate format.
ER Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
The assay system is all inclusive, providing reporter cells for each ER, optimized cell culture medium, a medium for diluting test compounds, 17-β-estradiol control agonist, stable-glow luciferase detection reagent, detailed protocol, Protocol Quick Guide, and a cell culture-ready assay plate in strip-well format so that (if preferred) ERα and ERβ assays may be performed at different times. The kit is shipped on dry ice.
| Product Description | Product Number |
|---|---|
| Human ER Reporter Assays PANEL | IB00421-48P |
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters. To initiate a Service Study, download and complete all fields of the Excel worksheet “Service Work Order" then submit the electronic file to INDIGO Customer Service.