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Cyn Monkey PGR Reporter Assay Kit

SIZE SKU PRICE
1 x-96 well format assays$860 USD
3 x-32 assays in-96 well format$930 USD
SIZE SKU
1 x-96 well format assays
3 x-32 assays in-96 well format
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Product Description and Product Data

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Cynomolgus Monkey Progesterone Receptor (cPGR). INDIGO’s cPGR reporter assay utilizes proprietary non-human mammalian cells that have been engineered to provide constitutive expression of the cPGR. In addition to cPGR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against cPGR. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.

Features

  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor, Nuclear Receptor Orthologs
SpeciesCyn Monkey
Receptor FormNative
Assay ModeAgonist, Antagonist
Kit Components
  • Monkey PGR Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • Progesterone, (ref. agonist; in DMSO)
  • Detection Substrate
  • Detection Buffer
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Shipping RequirementsDry Ice
Storage temperature-80C

Data

Agonist dose-response analyses of Monkey PGR. Agonist dose-response analyses of monkey PGR Reporter Cells using Progesterone (provided). In addition, to assess the level of background signal contributed by endogenously expressed PGR, or non-specific factors that may cause activation of the luciferase reporter gene, “mock” reporter cells were evaluated. (Mock reporter cells, which contain only the luciferase vector, are not provided with assay kits). The concentrated stock of progesterone was serially diluted in 2-fold decrements using CSM. Final assay concentrations for progesterone-treated cells ranged between 100 pM and 1.56 pM. Luminescence was quantified and average relative light units (RLU) and corresponding standard deviation (SD) and Fold-Activation values were determined for each treatment concentration (n = 3). Z’ values were calculated as described by Zhang, et al. (1999). Non-linear regression and EC50 analyses were performed using GraphPad Prism software.

Target Background

INDIGO’s Cyn Monkey Progesterone Receptor (cPGR) Reporter Assay System utilizes proprietary human cells engineered to provide constitutive, high-level expression of the full-length Cynomolgus Monkey Progesterone Receptor (nr3c3), a ligand-dependent transcription factor.

INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to a monkey PGR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in monkey PGR activity. Luciferase gene expression occurs after ligand-bound PGR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene.

Also available as a service

Progesterone Receptor (PGR, NR3C3)

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