Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Cynomolgus Monkey Peroxisome Proliferator-Activated Receptor Delta. INDIGO’s cPPAR delta reporter assay utilizes proprietary non-human mammalian cells that have been engineered to provide constitutive expression of the cPPAR delta. In addition to cPPAR delta Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against cPPAR delta. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
|Target Type||Nuclear Hormone Receptor, Nuclear Receptor Orthologs|
|Assay Mode||Agonist, Antagonist|
|Shelf Life||6 months|
|Shipping Requirements||Dry Ice|
INDIGO’s Cynomolgus Monkey PPAR delta (nr1c2; pparD/B; rPPARδ) Assay utilizes proprietary rodent cells engineered to provide high-level expression of cPPARδ. Reporter Cells also incorporate a responsive luciferase reporter gene, therefore, quantifying expressed luciferase activity provides a sensitive surrogate measure of cPPARδ activity in the treated cells. The principle application of this reporter assay system is in the screening of test samples to quantify functional activity, either agonist or antagonist, that they may exert against cynomolgus monkey PPARδ.
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