Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Estrogen Receptor Beta (ER). INDIGO’s ER Beta reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ER Beta. In addition to ER Beta Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human ER Beta. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
|Target Type||Nuclear Hormone Receptor|
|Assay Mode||Agonist, Antagonist|
|Shelf Life||6 months|
|Shipping Requirements||Dry Ice|
Estrogen receptor beta (ER-beta), also known as NR3A2 (nuclear receptor subfamily 3, group A, member 2), is a nuclear receptor which is activated by the sex hormone estrogen. ER beta is encoded by the human gene ESR2 (Estrogen Receptor 2). This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. ERβ is expressed by many tissues including blood monocytes and tissue macrophages, colonic and pulmonary epithelial cells and in prostatic epithelium and in malignant counterparts of these tissues. ERβ may have anti-proliferative effects and therefore oppose the actions of ERα in reproductive tissue. ERβ may also have an important role in adaptive function of the lung during pregnancy.
INDIGO’s Estrogen Receptor Beta Reporter Assay Systems utilize non-human mammalian cells engineered to express human ESR2, commonly referred to as NR3A2 or ERβ.
The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human estrogen receptor beta.
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