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Human ERRa Reporter Assay Kit

SIZES SKU
1 x 96-well format assays IB08001
3 x 32 assays in 96-well format IB08001-32
1 x 384-well format assays IB08002
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Product Description and Product Data

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Estrogen-Related Receptor Alpha (ERRa). INDIGO’s ERR Alpha reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ERR Alpha. In addition to ERR Alpha Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional inverse-agonist activities, that they may exert against human ERR Alpha. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.

Features

  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor
SpeciesHuman
Receptor FormHybrid
Assay ModeInverse Agonist
Kit Components
  • ERRa Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • XCT790, (ref. agonist; in DMSO)
  • Detection Substrate
  • Detection Buffer
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Orthologs AvailableNo
Shipping RequirementsDry Ice
Storage temperature-80C

Data

Inverse-agonist dose-response analyses of Human ERRα Human ERRα Reporter Cells were treated with varying concentrations of the inverse-agonist XCT790 (provided). Luminescence was quantified using a GloMax-Multi+ luminometer (Promega). Average relative light units (RLU) and corresponding standard deviation (SD) values were determined for each treatment concentration (n ≥ 6). Percent change in ERRα activity was calculated by normalizing respective RLU values from test compound-treated reporter cells to the RLU value of untreated reporter cells. Z’ values were calculated as described by Zhang, et al. (1999). Non-linear regression and IC50 determination were performed using GraphPad Prism software.

Target Background

INDIGO’s Estrogen-related Receptor Alpha assay systems utilize proprietary mammalian cells engineered to provide high-level expression of a hybrid form of Human ERRα (also known as NR3B1).

Reporter Cells also incorporate a responsive luciferase reporter gene. Quantifying expressed luciferase activity at the assay endpoint provides a sensitive surrogate measure of changes in ERRα activity in treated cells. As is true in vivo, these reporter cells express ERRα in a constitutive state of high-level activity.

Therefore, the principle application of this assay system is in the screening of test samples to quantify inverse-agonist activities that they may exert against human ERRα.

Also available as a service

Estrogen-related Receptor Alpha (ERRa, NR3B1)

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