Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Retinoid X Receptor alpha (RXRa). INDIGO’s RXR Alpha reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the RXR Alpha. In addition to RXR Alpha Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human RXR Alpha. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
|Target Type||Nuclear Hormone Receptor|
|Assay Mode||Agonist, Antagonist|
|Shelf Life||6 months|
|Shipping Requirements||Dry Ice|
Retinoid X receptor alpha (RXRα), also known as NR2B1 is a nuclear receptor which in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcription factors.
INDIGO’s RXR Alpha Reporter Assay System utilizes mammalian cells engineered to provide constitutive, high-level expression of Human Retinoid X Receptor Alpha (NR2B1), a ligand-dependent transcription factor commonly referred to as RXRA or RXRα. Additionally, these cells contain an RXRα-responsive luciferase reporter gene. Thus, quantifying luciferase activity provides a surrogate measure of RXRα activity in the treated reporter cells.
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