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Human RXRβ Reporter Assay Kit

1 x-96 well format assays
3 x-32 assays in-96 well format
1 x-384 well format assays
1 x-96 well format assays
3 x-32 assays in-96 well format
1 x-384 well format assays

Product Description and Product Data

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Retinoid X Receptor Beta (RXRb). INDIGO’s RXR Beta reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the RXR Beta. In addition to RXR Beta Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human RXR Beta. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.


  • Ready to Use Upon Receipt

  • Includes All Needed Components
  • Contains Transfected Reporter Cells
  • Eliminates Cell Licensing Fees
  • Clear, Reproducible Results
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor
Receptor FormHybrid
Assay ModeAgonist, Antagonist
Kit Components
  • RXRb Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • 9-cis Retinoic Acid, (ref. agonist; in DMSO)
  • Detection Substrate
  • Detection Buffer
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Orthologs AvailableNo
Shipping RequirementsDry Ice
Storage temperature-80C


Agonist and Antagonist dose-response analyses of the Human RXRβ. A.) Analyses of RXRβ Reporter Cells using the reference agonists 9-cis-Retinoic Acid (provided), and LG100268 (Sigma-Aldrich). B.) Analyses of RXRβ antagonist dose-responses using HX531 and UVI3003 (Tocris). Assay setups and quantification of RXRβ activity were performed following the protocol provided in the assay Technical Manual. Luminescence was quantified and average relative light units (RLU) and corresponding standard deviation (SD) values were determined for each treatment concentration (n ≥ 4). Values of Fold-activation and Z’ were calculated as described by Zhang, et al. (1999). Non-linear regression and EC50 analyses were performed using GraphPad Prism software. High S/B and Z' scores confirm the robust performance of this RXRβ Assay.

Target Background

Retinoid X receptor beta (RXR-beta), also known as NR2B2 is a nuclear receptor which in humans is encoded by the RXRB gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcription factors.

INDIGO’s Human Retinoid X Receptor Beta (RXRβ) Reporter Assay System utilizes proprietary mammalian cells engineered to provide constitutive, high-level expression of human RXRB (NR2B2), a ligand-dependent transcription factor. Because these cells incorporate a responsive luciferase reporter gene, quantifying expressed luciferase activity provides a sensitive surrogate measure of RXRβ activity in treated cells. The primary application of this reporter assay system is in the screening of test samples to quantify functional activity, either agonist or antagonist, that they may exert against human RXRβ.

Also available as a service

Retinoid X Receptor Beta (RXRb, NR2B2)

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