Panel of Human PPAR Reporter Assays: PPARa, PPARd, & PPARg
Product Description and Product Data
INDIGO’s Human PPAR Panel (PPAR alpha, PPAR delta, and PPAR gamma) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to reporter cells for each PPAR, optimized growth media, media for diluting test compounds, a control agonist for each PPAR, luciferase detection reagent, detailed protocol, and a protocol quick guide, and a cell culture-ready assay plate in strip-well format so that (if preferred) PPAR alpha, PPAR delta, and PPAR gamma assays may be performed at different times. This panel contains sufficient materials to perform 32 PPAR alpha assays, 32 PPAR gamma assays, and 32 PPAR delta assays, all in a single 96-well plate format.
Features
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
Product Specifications
| Target Type | Nuclear Hormone Receptor | ||
| Species | Human | ||
| Receptor Form | Hybrid | ||
| Assay Mode | Agonist, Antagonist | ||
| Kit Components |
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| Shelf Life | 6 months | ||
| Shipping Requirements | Dry Ice | ||
| Storage temperature | -80C |
Data
Target Background
PPAR ligands, whose names derived from their effect on inducing an increase in the size and number of peroxisomes, include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified.
This PANEL of PPAR Assays utilizes non-human mammalian cells engineered to express the human PPAR proteins: PPARα (NR1C1), PPARδ (NR1C2), and PPARγ (NR1C3).
Product Documentation
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