Product Description and Product Data
INDIGO’s Retinoic Acid Receptors Panel (RAR alpha, RAR beta, and RAR gamma) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to reporter cells for each RAR, optimized cell culture medium, a medium for diluting test compounds, a control agonist for each RAR, stable-glow luciferase detection reagent, detailed protocol, Protocol Quick Guide, and a cell culture-ready assay plate in strip-well format so that (if preferred) RAR alpha, RAR beta, and RAR gamma assays may be performed at different times. This panel contains sufficient materials to perform 32 RAR alpha assays, 32 RAR beta assays, and 32 RAR gamma assays in 96-well plate format.
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
|Target Type||Nuclear Hormone Receptor|
|Assay Mode||Agonist, Antagonist|
|Shelf Life||6 months|
|Shipping Requirements||Dry Ice|
Retinoic acid receptors (RARs) are nuclear hormone receptors of the NRB1 class, which function as heterodimers with retinoid X receptors (RXRs). There are three distinct RAR subtypes; RARalpha, RARbeta and RARgamma. RARalpha is present in most tissue types, whereas RARbeta and RARgamma expression is more selective. RXR-RAR heterodimers act as ligand-dependent transcriptional regulators by binding to the specific retinoic acid response element (RARE) found in the promoter regions of target genes. In the absence of an RAR agonist, RXR-RAR recruits co-repressor proteins such as NCoR and associated factors such as histone deacetylase to maintain a condensed chromatin structure. RAR agonist binding stimulates co-repressor release and co-activator complexes, such as histone acetyltransferase, are recruited to activate transcription. RARs transduce retinoid signals in vivo, which mediates proper embryogenesis, differentiation and growth arrest. Specifically, RXRalpha-RARgamma heterodimers are necessary for growth arrest and viseral and primitive endodermal differentiation, whereas RXRalpha-RARalpha is required for cAMP-dependent parietal endodermal differentiation. In vitro it has been difficult to elucidate the roles of individual subtypes as functional RAR knockouts generate artificial redundancies that are thought not to exist under normal conditions.
This PANEL of Retinoic Acid Receptors Reporter Assays utilizes non-human mammalian cells engineered to express distinct human RARα (NR1B1), RARβ (NR1B2), and RARγ (NR1B3) proteins.
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