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Panel of Human RXR Reporter Assays: RXRa, RXRb & RXRg

SIZES SKU
Each in 1 x 32 96-well format IB00831-32P
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Product Description and Product Data

INDIGO’s Retinoid X Receptors Panel (RXR alpha, RXR beta, and RXR gamma) assay kit is an all-inclusive firefly luciferase reporter assay system that includes in addition to reporter cells for each RXR, optimized cell culture medium, a medium for diluting test compounds, a control agonist for each RXR, stable-glow luciferase detection reagent, detailed protocol, Protocol Quick Guide, and a cell culture-ready assay plate in strip-well format so that (if preferred) RXR alpha, RXR beta, and RXR gamma assays may be performed at different times. This panel contains sufficient materials to perform 32 RXR alpha assays, 32 RXR beta assays, and 32 RXR gamma assays in 96-well plate format.

Features

  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeNuclear Hormone Receptor
SpeciesHuman
Receptor FormHybrid
Assay ModeAgonist, Antagonist
Kit Components
  • RXRa Reporter Cells
  • RXRb Reporter Cells
  • RXRg Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • 9 cis-Retinoic Acid, (ref. agonist RAR; in DMSO)
  • Detection Substrate
  • Detection Buffer
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Orthologs AvailableNo
Shipping RequirementsDry Ice
Storage temperature-80C

Data

Target Background

Retinoid X receptors (RXRs) and retinoic acid receptors (RARs), are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors exert their action by binding, as homodimers or heterodimers, to specific sequences in the promoters of target genes and regulating their transcription. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcription factors.

This PANEL of Retinoid X Receptors Reporter Assays utilizes non-human mammalian cells engineered to express distinct human RXRα (NR2B1), RXRβ (NR2B2), and RXRγ (NR2B3) proteins.

Citations

Preclinical assessment of dual CYP26[A1/B1] inhibitor, DX308, as an improved treatment for keratinization disorders

Retinoid‐based therapies are commonly used in the treatment of disorders of keratinization and other skin disorders but can result in non‐specific effects and adverse reactions. Use of retinoic acid metabolism blocking agents (RAMBAs) such as DX308 may address these shortcomings.
2021-03-26

Tri-modal Therapy: Combining Hyperthermia with Repurposed Bexarotene and Ultrasound for Treating Liver Cancer

Repurposing of existing cancer drugs to overcome their physical limitations, such as insolubility, represents an attractive strategy to achieve enhanced therapeutic efficacy and broaden the range of clinical applications. Such an approach also promises to offer substantial cost savings in drug development efforts. Here we use repurposed FDA-approved topical agent bexarotene (Targretin™), currently in limited use for cutaneous manifestations of T-cell lymphomas, and re-engineer it for use in solid tumor applications by forming self-assembling nanobubbles. Physicochemical characterization studies of the novel prodrug nanobubbles demonstrated their stability, enhanced target cell-internalization capability and highly controlled release profile in response to application of focused ultrasound energy. Using an in vitro model of hepatocellular carcinoma and an in vivo large animal model of liver ablation, we demonstrate the effectiveness of bexarotene prodrug nanobubbles when used in conjunction with catheter-based ultrasound, thereby highlighting the therapeutic promise of this trimodal approach.
2015-10-12

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Retinoid X Receptor Alpha, Beta, & Gamma (RXRa, RXRb, & RXRg)

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