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Zebrafish ERa Reporter Assay Kit
Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Zebrafish Estrogen Receptor alpha. INDIGO’s zER alpha reporter assay utilizes proprietary non-human cells that have been engineered to provide constitutive expression of the zER alpha. In addition to zER alpha Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against zER alpha. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Features
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
Product Specifications
| Target Type | Nuclear Hormone Receptor, Nuclear Receptor Orthologs | ||
| Species | Zebrafish | ||
| Receptor Form | Native | ||
| Assay Mode | Agonist, Antagonist | ||
| Kit Components |
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| Shelf Life | 6 months | ||
| Shipping Requirements | Dry Ice | ||
| Storage temperature | -80C |
Data
Target Background
INDIGO’s Zebrafish Estrogen Receptor Alpha Reporter Assay System utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of Zebrafish (Danio rerio) Estrogen Receptor Alpha 1 (nr3a1), a ligand-dependent transcription factor.
INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to an ERα-responsive promoter. Thus, quantifying changes in luciferase expression in the test sample-treated reporter cells provides a sensitive surrogate measure of changes in zERα activity. Luciferase gene expression occurs after ligand-bound zERα undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators, and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo.
The principal application of this assay is in the screening of test samples to quantify any functional bioactivity that they may exert against zebrafish AR. In particular, zebrafish reporter assays are frequently used in the monitoring of environmental samples for the presence of biohazardous chemical pollutants, such as endocrine disruptors.
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