Sub-Total: $0.00 USD
Have questions about this assay kit?
Mouse PXR Reporter Assay Kit
Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Mouse Pregnane X Receptor (mPXR). INDIGO’s mPXR reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the mPXR. In addition to mPXR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against mPXR. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Features
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
Product Specifications
| Target Type | Nuclear Hormone Receptor, Nuclear Receptor Orthologs | ||
| Species | Mouse | ||
| Receptor Form | Hybrid | ||
| Assay Mode | Agonist, Antagonist | ||
| Kit Components |
| ||
| Shelf Life | 6 months | ||
| Shipping Requirements | Dry Ice | ||
| Storage temperature | -80C |
Data
Target Background
Pregnane X Receptor also known as nuclear receptor subfamily 1, group I, member 2 (NR1I2) and SXR is a nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. PXR activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs, and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones, its response to specific ligands is species-specific. PXR is activated by naturally occurring steroids, such as pregnenolone and progesterone and binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
This assay product utilizes proprietary mammalian cells engineered to provide constitutive, high-level expression of the Mouse Pregnane X Receptor (nr1i2), a ligand-dependent transcription factor commonly referred to as PXR. PXR is also known as the Steroid and Xenobiotic sensing nuclear receptor (SXR).
Citations
Microbiome metabolism of dietary phytochemicals controls the anticancer activity of PI3K inhibitors
Roichman, Zuo, Hwang et al
Cell (2025) 188 (11), 3065-3080.e21 DOI: 10.1016/j.cell.2025.04.041
Roichman, Zuo, Hwang et al
Cell (2025) 188 (11), 3065-3080.e21 DOI: 10.1016/j.cell.2025.04.041
Cited by 35
Snippet: ouse PXR Reporter Assay Kit Indigo Biosciences Cat# M07001-32 Mouse CAR Reporter Assay Kit Indigo Biosciences Cat# M00901-32 Ultra Sensitive Mouse Insulin ELISA Kit Crystal Chem Cat# 90080
Evidence for triclosan-induced activation of human and rodent xenobiotic nuclear receptors
Paul, Thompson, Simmons et al
Toxicol In Vitro (2013) 27 (7), 2049-60 DOI: 10.1016/j.tiv.2013.07.008
Paul, Thompson, Simmons et al
Toxicol In Vitro (2013) 27 (7), 2049-60 DOI: 10.1016/j.tiv.2013.07.008
Cited by 52
Snippet: er;(2) a firefly luciferase reporter plasmid driven by the UAS GAL4 DNA response element;and, (3) a Renilla luciferase reporter gene (pRL-luciferase;Promega;Madison, WI) as a transfection efficiency and cell viability control. These assays were conducted by Indigo Biosciences, and the use and validation of similar systems has been reported previously (Gillies et al., 2012, Tien et al., 2006, Vanden Heuvel et al., 2006). … Additionally, the RPXR results were confirmed in this second laboratory (INDIGO Biosciences). …
Triclosan Disrupts Thyroid Hormones: Mode-of-Action, Developmental Susceptibility, and Determination of Human Relevance
Paul
Thesis (2011)
Paul
Thesis (2011)
Snippet: Cell-based receptor-reporter assays in human embryonic kidney cells (HEK293T) (INDIGO BioSciences) were used to evaluate the ligand-binding potential of TCS for all three active human CAR splice variants (hCAR1, hCAR2, hCAR3), rat CAR, mouse CAR, and human, rat, and mouse PXR
Showing 1-3 of 3 papers.
Powered by BizGenius
Also available as a service