Androgen Receptor (AR; NR3C4)
Also available in: Rat
This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full length, unmodified Human Androgen Receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR.
INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to a
The androgen receptor is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression; however, the androgen receptor has other functions as well.
Androgen regulated genes are critical for the development and maintenance of the male sexual phenotype. In some cell types testosterone interacts directly with androgen receptors while in others testosterone is converted by 5-alpha-reductase to dihydrotestosterone, an even more potent agonist for androgen receptor activation. Testosterone appears to be the primary androgen receptor activating hormone in the Wolffian duct while dihydrotestosterone is the main androgenic hormone in the urogenital sinus, urogenital tubercle, and hair follicles. Hence testosterone is primarily responsible for the development of male primary sexual characteristics while dihydrotestosterone is responsible for secondary male characteristics.
For more information on AR, visit the Nuclear Receptor Resource.
Also available in: Rat
Kits are offered in different assay formats to accommodate researchers' needs: 3x 32, 1x 96, and 1x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.
AR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human androgen receptor. This kit product is an all-inclusive assay system that includes, in addition to AR Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
| Product Family | Product Number | Product Description |
| IB0300 AR (NR3C4) | IB03001-32 | Human AR Reporter Assay System, 3 x 32 assays in 96-well format |
| IB03001 | Human AR Reporter Assay System, 1 x 96-well format assays | |
| IB03002 | Human AR Reporter Assay System, 1 x 384-well format assays |
Service Assays: Human, >a href="https://indigobiosciences.com/ortholog-nr-assays/rat-androgen-receptor-rar-nr3c4/">Rat
The primary application of INDIGO's cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and 'vehicle' control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online "Request a Quote" form or contact an INDIGO Customer Service Representative to discuss your desired study parameters. To initiate a Service Study, download and complete all fields of the Excel worksheet "Service Work Order" then submit the electronic file to INDIGO Customer Service.
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An Automated Cell-Based Platform for the Rapid Detection of Novel Androgen Receptor Modulators
View Full Size Research conducted by: Brad Larson (1), Bruce Sherf (2), & Peter Banks (1) (1)BioTek Instruments, Inc., Winooski, VT, USA (2) INDIGO Biosciences, Inc., State College, PA, USA Date of Publication: March 2012
An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators
ABSTRACT The Androgen Receptor (AR) is a member of the family of nuclear receptors responsive to steroid hormones. AR activity is regulated through the binding of androgenic hormones (eg., testosterone and di-hydroxy testosterone). In turn, AR functions to regulate the expression of a myriad of genes involved in metabolic processes, cell proliferation/apoptosis, and male sexual differentiation and development. AR dysfunction often hasRead More