Product Description and Product Data
This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Estrogen-Related Receptor Beta (ERRb). INDIGO’s ERR Beta reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the ERR Beta. In addition to ERR Beta Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify inverse-agonist or agonist activities that they may exert against human ERR Beta. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.
Features
Clear, Reproducible Results
- All-Inclusive Assay Systems
- Exceptional Cell Viability Post-Thaw
- Consistent Results Lot to Lot
Product Specifications
Target Type | Nuclear Hormone Receptor | ||
Species | Human | ||
Receptor Form | Hybrid | ||
Assay Mode | Inverse Agonist | ||
Kit Components |
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Shelf Life | 6 months | ||
Orthologs Available | No | ||
Shipping Requirements | Dry Ice | ||
Storage temperature | -80C |
Data
Target Background
INDIGO’s Estrogen-related Receptor assay utilizes proprietary human mammalian cells engineered to provide high-level expression of a hybrid form of the Human Estrogen-Related Receptor Beta (NR3B2).
Reporter Cells also incorporate a responsive luciferase reporter gene. Quantifying expressed luciferase activity at the assay endpoint provides a sensitive surrogate measure of changes in ERRβ activity in treated cells. As is true in vivo, these reporter cells express ERRβ in a constitutive state of high-level activity.
Therefore, the principal application of this assay is in the screening of test samples to quantify inverse-agonist or agonist activities that they may exert against human ERRβ.
Also available as a service