Liver X Receptor Alpha (LXRα; NR1H3)
|Product Family||Product Number||Product Description||Technical Manual|
|IB00311-32||Human LXRα Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|IB00311||Human LXRα Reporter Assay System, 1 x 96-well format assays||Technical Manual|
|IB00312||Human LXRα Reporter Assay System, 1 x 384-well format assays||Technical Manual|
Liver X Receptor Alpha Assay Kit
This Liver X Receptor Alpha (LXRα) assay kit is an all-inclusive LXR alpha reporter assay system that includes, in addition to LXRα Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
LXRα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s LXR alpha assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
INDIGO's LXRα assay kits are offered in different assay formats to accommodate researchers’ needs: 3 x 32, 1 x 96, and 1 x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
LXRa assay kit also available in: Mouse
Liver X Receptor Alpha Assay Services
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
LXRa Service Assays Available: Human, Mouse
Liver X Receptor Alpha Background
Liver X Receptor Alpha (LXRα) is a nuclear receptor protein encoded by the NR1H3 gene (nuclear receptor subfamily 1, group H, member 3). The Liver X Receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with Retinoid X Receptors and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
These LXRα Reporter Assay Systems utilize non-human mammalian cells engineered to express human NR1H3 protein, commonly referred to as LXRα.
The principle application of this LXRα assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human liver x receptor alpha.
For more information on LXRα, visit the Nuclear Receptor Resource.
Synonyms: Liver X Receptor Alpha, LXRα, LXRa, LXR alpha, Human Liver X Receptor Alpha, Human LXR alpha, NR1H3
Liver X Receptor Alpha Assay Data
Human LXRα dose-response assays. A.) Agonist assays. LXRα Reporter Cells were treated with the reference agonists T0901317 (provided), GW3965 (Tocris), or Acetyl Podocarpic Acid (ADP; Cayman Chemical). B.) Antagonist assays. Reporter Cells were treated with the reference antagonists GSK 2033 (Tocris) or SR 9243 (Cayman Chemical). Luminescence was quantified and values of Average RLU and corresponding standard deviation and percent coefficient of variation, and Fold-Activation were calculated for each treatment group (n=4). Z’ values were calculated as described by Zhang, et al. (1999). Non-linear regression and EC50 analyses were performed using GraphPad Prism software.