Liver X Receptor Beta (LXRβ; NR1H2)
|Product Family||Product Number||Product Description||Technical Manual|
|IB00301-32||Human LXRβ Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|IB00301||Human LXRβ Reporter Assay System, 1 x 96-well format assays||Technical Manual|
|IB00302||Human LXRβ Reporter Assay System, 1 x 384-well format assays||Technical Manual|
Liver X Receptor Beta Assay Kit
This Liver X Receptor Beta (LXRβ) assay kit is an all-inclusive LXR beta reporter assay system that includes, in addition to LXRβ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, a Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
INDIGO’s cell-based reporter assays allow scientists to detect any biological activity that their test samples may exert against a specific receptor present in the cell. They utilize firefly luciferase reporter gene technology which provides superior precision and sensitivity. Since the receptor binding controls the expression of the luciferase reporter gene, luciferase activity in the cells can be correlated directly with the activity of the receptor. The strength of an interaction of a chemical with the target receptor is quantified using a luminometer to measure the level of light emitted.
Fast, reproducible, easy-to-analyze results are only four steps away
Many luciferase reporter assays require the user to grow their own cells and take time to optimize the results. INDIGO’s reporter cells contain the receptor of interest and the luciferase reporter gene. Reporter cells have been optimized to provide extreme sensitivity to quantify even small changes in receptor activity. With INDIGO’s cell-based reporter assays, the process is as easy as Thaw, Feed, Dose, and Read.
Liver X Receptor Beta Reporter Cells
LXRβ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This proprietary cryopreservation process, enables long-term preservation of our unique reporter cells, so we can ship our cryopreserved reporter cells and assay reagents to you via overnight delivery, for your immediate use. Or, you can store the assay kits at -80°C. Once thawed, reporter cells are ready for immediate use so there is no need to take time on intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
Liver X Receptor Beta Luciferase Reporter
INDIGO’s LXRβ assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
LXRβ assay kit also available in: Rat, Mouse
Liver X Receptor Beta Assay Services
The primary application of INDIGO’s cell-based reporter assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
LXRb Service Assays: Human, Rat, Mouse
Liver X Receptor Beta Background
Liver X receptor β (LXRβ) is a member of the nuclear receptor family of transcription factors. LXRβ is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with the Retinoid X Receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
These LXRβ Reporter Assay Systems utilize proprietary mammalian cells engineered to express human NR1H2 protein, commonly referred to as LXRβ.
The principle application of this LXRβ assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human liver x receptor beta.
For more information on LXRβ, visit the Nuclear Receptor Resource.
Synonyms: Liver X Receptor Beta, LXRβ, LXRb, LXR beta, Human Liver X Receptor Beta, Human LXR beta, NR1H2
Liver X Receptor Beta Assay Data
Dose-response analyses of the Human LXRβ. A.) Agonist dose-response analyses. LXRβ Reporter Cells were treated with a dilutions series of the reference agonists T0901317 (provided), APD (Cayman Chem.) or GW3965 (Tocris). B.) Antagonist dose-response analyses performed by co-treating reporter cells with a fixed (EC80) concentration or TO901317 and varying concentrations of the reference antagonists GSK 2033 or SR 9243 (Tocris). Luminescence was quantified and average relative light units (RLU) and corresponding values of standard deviation (SD) were determined for each treatment concentration (n = 3). Fold-Activation and Z’ values were calculated as described by Zhang, et al. (1999). Non-linear regression and EC50 / IC50 analyses were performed using GraphPad Prism software.
Liver X Receptor Beta Assay Research Areas
Diabetes; Dyslipidemia; Artherosclerosis; CNS, Circadian & Basal Metabolism; Metabolic Disease; NASH/NAFLD