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  • HOME
  • ABOUT
    • About
    • Why INDIGO
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      • Management
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    • Ortholog Assays
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    • In Vitro Toxicology Platform
    • MDR1 / Human P-Glycoprotein
    • Gene Expression
    • NASH Nuclear Receptors For Research
    • Disease States
    • Live Cell Multiplex
    • Custom Assay Development
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Liver X Receptor Beta (LXRβ; NR1H2)

Product Family Product Number Product Description Technical Manual
IB0030
LXRβ
(NR1H2)
IB00301-32 Human LXRβ Reporter Assay System, 3 x 32 assays in 96-well format Technical Manual
IB00301 Human LXRβ Reporter Assay System, 1 x 96-well format assays Technical Manual
IB00302 Human LXRβ Reporter Assay System, 1 x 384-well format assays Technical Manual
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This Liver X Receptor Beta (LXRβ) kit is an all-inclusive assay system that includes, in addition to LXRβ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, a Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.

LXRβ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.

INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.

Kits are offered in different assay formats to accommodate researchers’ needs: 3 x 32, 1 x 96, and 1 x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.

Liver X Receptor Beta agonist

Assay Kit & Platforms

Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.

Also available in: Rat, Mouse

Liver X receptor β (LXRβ) is a member of the nuclear receptor family of transcription factors. LXRβ is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with the Retinoid X Receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.

These LXRβ Reporter Assay Systems utilize proprietary mammalian cells engineered to express human NR1H2 protein, commonly referred to as LXRβ.

The principle application of this LXRβ assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human liver x receptor beta.

For more information on LXRβ, visit the Nuclear Receptor Resource.

Service Assays: Human, Rat, Mouse

The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.

Diabetes; Dyslipidemia; Artherosclerosis; CNS, Circadian & Basal Metabolism; Metabolic Disease; NAFLD/NASH

Bergapten inhibits liver carcinogenesis by modulating LXR/PI3K/Akt and IDOL/LDLR pathways

ABSTRACT Oxysterol receptors LXRs (α and β) are recently reported to be one of the novel and potential therapeutic targets in reducing cell proliferation and tumor growth in different system model. Activation of LXRs is correlated with modification of PI3K/Akt pathway. LXRs are also found to play a critical role in maintaining lipid homeostatais byRead More

Posted in New Publications | Tagged Bergapten, Hepatocellular carcinoma, IDOL, LDLR, lipid metabolism, LXR, LXRα, LXRβ, PI3K | Comments Off on Bergapten inhibits liver carcinogenesis by modulating LXR/PI3K/Akt and IDOL/LDLR pathways

Differential modulation of FXR activity by chlorophacinone and ivermectin analogs

ABSTRACT Chemicals that alter normal function of farnesoid X receptor (FXR) have been shown to affect the homeostasis of bile acids, glucose, and lipids. Several structural classes of environmental chemicals and drugs that modulated FXR transactivation were previously identified by quantitative high-throughput screening (qHTS) of the Tox21 10 K chemical collection. In the present study, weRead More

Posted in New Publications | Tagged CAR, FXR, LXR, LXRα | Comments Off on Differential modulation of FXR activity by chlorophacinone and ivermectin analogs

Effects of Munitions Compounds on Xenobiotic-Activated Nuclear Receptors and Signaling Pathways

ABSTRACT Exposure to certain munitions compounds is know to alter physiological functions in test organisms, however little is known about their molecular and cellular effects. Several nuclear receptors are regulated by xenobiotic compounds. These nuclear receptors belong to a class of ligand-activated transcription factors that, when heterodimerized with RXRa and bound to their respective DNARead More

Posted in New Publications | Tagged CAR, FXR, LXR, PPAR | Comments Off on Effects of Munitions Compounds on Xenobiotic-Activated Nuclear Receptors and Signaling Pathways

Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes

ABSTRACT The apolipoprotein E (APOE) gene is the most highly associated susceptibility locus for late onset Alzheimer’s Disease (AD), and augmenting the beneficial physiological functions of apoE is a proposed therapeutic strategy. In a high throughput phenotypic screen for small molecules that enhance apoE secretion from human CCF-STTG1 astrocytoma cells, we show the chrysanthemic esterRead More

Posted in New Publications | Tagged LXR | Comments Off on Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes

Indigo Announces In Vitro Toxicology Platform

In vitro toxicology platform provides predictive model of liver toxicity.  Aims to reduce the high rates of drug­-induced liver damage State College, PA (May 4, 2016) INDIGO Biosciences, the recognized industry leader in nuclear receptor research, has completed development of an in vitro toxicology platform, meeting the demand for predictive liver toxicity models.  INDIGO’s inRead More

Posted in press | Tagged AhR, CAR, In Vitro Toxicology, LRH-1, LXR, Nrf2, PPAR, PXR | Comments Off on Indigo Announces In Vitro Toxicology Platform

The Flavone Luteolin Inhibits Liver X Receptor Activation

ABSTRACT Luteolin is a dietary flavonoid with medicinal properties including antioxidant, antimicrobial, anticancer, antiallergic, and anti-inflammatory. However, the effect of luteolin on liver X receptors (LXRs), oxysterol sensors that regulate cholesterol homeostasis, lipogenesis, and inflammation, has yet to be studied. To unveil the potential of luteolin as an LXRα/β modulator, we investigated by real-time RT-PCRRead More

Posted in New Publications | Tagged LXR, LXRα, LXRβ | Comments Off on The Flavone Luteolin Inhibits Liver X Receptor Activation

In vitro assessment of human nuclear hormone receptor activity and cytotoxicity of the fame retardant mixture FM 550 and its triarylphosphate and brominated components

ABSTRACT Firemaster®550 (FM 550) is a mixture of brominated and triarylphosphate flame retardants used in polyurethane foam-based products. The primary components are also used in numerous other applications and are thus common household and industrial contaminants. Our previous animal studiessuggested that FM 550 exposure may alter metabolism and cause weight gain. Employing human nuclear receptorRead More

Posted in New Publications | Tagged LXR, PPAR, PPARγ, TR | Comments Off on In vitro assessment of human nuclear hormone receptor activity and cytotoxicity of the fame retardant mixture FM 550 and its triarylphosphate and brominated components

Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil

ABSTRACT Omega-3-PUFAs, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), are associated with prevention of various aspects of metabolic syndrome. In the present studies, the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other  3-PUFAs. The EPA-rich oil (EO) altered the expression of FA metabolism genes in THP-1 cells, including stearoylRead More

Posted in New Publications | Tagged LXR, PPAR, PPARα, PPARβ, PPARγ, RXR | Comments Off on Regulation of inflammatory and lipid metabolism genes by eicosapentaenoic acid-rich oil

Differential Activation of Nuclear Receptors by Perflunoriated Fatty Acid Analogs and Natural Fatty Acids: A Comparison of Human, Mouse, and Rat Peroxisome Proliferator-Activated Receptor Receptor-a, -b, and -c, Liver X Receptor-b, and Retinoid X Receptor-a

ABSTRACT Administration of ammonium salts of perfluorooctanoate (PFOA) to rats results in peroxisome proliferation and benign liver tumors, events associated with activation of the nuclear receptor (NR) peroxisome proliferator-activated receptor-a (PPARa). Due to its fatty acid structure, PFOA may activate other NRs, such as PPARb, PPARg, liver X receptor (LXR), or retinoid X receptor (RXR). In this study, theRead More

Posted in New Publications | Tagged LXR, LXRβ, PPAR, PPARα, PPARβ, PPARγ, RXR | Comments Off on Differential Activation of Nuclear Receptors by Perflunoriated Fatty Acid Analogs and Natural Fatty Acids: A Comparison of Human, Mouse, and Rat Peroxisome Proliferator-Activated Receptor Receptor-a, -b, and -c, Liver X Receptor-b, and Retinoid X Receptor-a
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