Liver X Receptor Beta (LXRβ; NR1H2)
These LXRβ Reporter Assay Systems utilize non-human mammalian cells engineered to express human NR1H2 protein, commonly referred to as LXRβ.
Liver X receptor β (LXRβ) is a member of the nuclear receptor family of transcription factors. LXRβ is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with the Retinoid X Receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
For more information on LXRβ, visit the Nuclear Receptor Resource.
Kits are offered in different assay formats to accommodate researchers’ needs: 3 x 32, 1 x 96, and 1 x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.
LXRβ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
The principle application of this LXRβ assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human liver x receptor beta. This kit product is an all-inclusive assay system that includes, in addition to LXR Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, a Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
|Product Family||Product Number||Product Description|
|IB0030 LXRβ (NR1H2)||IB00301-32||Human LXRβ Reporter Assay System, 3 x 32 assays in 96-well format|
|IB00301||Human LXRβ Reporter Assay System, 1 x 96-well format assays|
|IB00302||Human LXRβ Reporter Assay System, 1 x 384-well format assays|
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters. To initiate a Service Study, download and complete all fields of the Excel worksheet “Service Work Order" then submit the electronic file to INDIGO Customer Service.
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In vitro assessment of human nuclear hormone receptor activity and cytotoxicity of the fame retardant mixture FM 550 and its triarylphosphate and brominated components
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Differential Activation of Nuclear Receptors by Perflunoriated Fatty Acid Analogs and Natural Fatty Acids: A Comparison of Human, Mouse, and Rat Peroxisome Proliferator-Activated Receptor Receptor-a, -b, and -c, Liver X Receptor-b, and Retinoid X Receptor-a
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