Liver X Receptor Beta (LXRβ; NR1H2)
|Product Family||Product Number||Product Description||Technical Manual|
|IB00301-32||Human LXRβ Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|IB00301||Human LXRβ Reporter Assay System, 1 x 96-well format assays||Technical Manual|
|IB00302||Human LXRβ Reporter Assay System, 1 x 384-well format assays||Technical Manual|
Liver X receptor β (LXRβ) is a member of the nuclear receptor family of transcription factors. LXRβ is encoded by the NR1H2 gene (nuclear receptor subfamily 1, group H, member 2). The Liver X receptors (LXRs) were originally identified as orphan members of the nuclear receptor superfamily because their ligands were unknown. Like other receptors in the family, LXRs heterodimerize with the Retinoid X Receptor and bind to specific response elements (LXREs) characterized by direct repeats separated by 4 nucleotides.
These LXRβ Reporter Assay Systems utilize proprietary mammalian cells engineered to express human NR1H2 protein, commonly referred to as LXRβ.
The principle application of this LXRβ assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human liver x receptor beta.
For more information on LXRβ, visit the Nuclear Receptor Resource.
Synonyms: Liver X Receptor Beta, LXRβ, LXRb, LXR beta, Human Liver X Receptor Beta, Human LXR beta, NR1H2
This Liver X Receptor Beta (LXRβ) kit is an all-inclusive assay system that includes, in addition to LXRβ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, a Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
LXRβ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Kits are offered in different assay formats to accommodate researchers’ needs: 3 x 32, 1 x 96, and 1 x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.