Peroxisome Proliferator-Activated Receptor Beta/Delta (PPARδ/PPARβ; NR1C2)
|Product Family||Product Number||Product Description||Technical Manual|
|IB00121-32||Human PPARδ Reporter Assay System, 3 x 32 assays in 96-well format||Technical Manual|
|IB00121||Human PPARδ Reporter Assay System, 1 x 96-well format assays||Technical Manual|
|IB00122||Human PPARδ Reporter Assay System, 1 x 384-well format assays||Technical Manual|
This Peroxisome Proliferator-Activated Receptor Beta/Delta (PPARδ/PPARβ) kit is an all-inclusive assay system that includes, in addition to PPARδ/PPARβ Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
PPARδ/PPARβ Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32, 1x 96, and 1x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
Peroxisome proliferator-activated receptor delta (PPARδ or PPARβ), also known as NR1C2 is a nuclear receptor encoded by the PPARδ gene. This protein is a potential inhibitor of ligand-induced transcription activity of PPARα and PPARγ. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of PPARδ gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein involved in the APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of PPARδ in myelination of the corpus callosum, epidermal cell proliferation, and glucose and lipid metabolism. PPARδ has been shown to be involved in differentiation, lipid accumulation, directional sensing, polarization, and migration in keratinocytes.
INDIGO's PPARδ Reporter Assay Systems utilize proprietary mammalian cells engineered to express human PPARδ.
The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human peroxisome proliferator-activated receptor delta.
For more information on PPARδ, visit the Nuclear Receptor Resource.
Service Assays: Human, Mouse, Rat, Cyn Monkey, Dog
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
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