Peroxisome Proliferator-Activated Receptor Beta/Delta (PPARδ/PPARβ; NR1C2)
These PPARδ Reporter Assay Systems utilize non-human mammalian cells engineered to express human PPARδ.
Peroxisome proliferator-activated receptor delta (PPARδ or PPARβ), also known as NR1C2 is a nuclear receptor encoded by the PPARδ gene. This protein is a potential inhibitor of ligand-induced transcription activity of PPARα and PPARγ. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of PPARδ gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein involved in the APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of PPARδ in myelination of the corpus callosum, epidermal cell proliferation, and glucose and lipid metabolism. PPARδ has been shown to be involved in differentiation, lipid accumulation, directional sensing, polarization, and migration in keratinocytes.
For more information on PPARδ, visit the Nuclear Receptor Resource.
Kits are offered in different assay formats to accommodate researchers’ needs: 3x 32, 1x 96, and 1x 384 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications. Assay systems are all inclusive, providing reporter cells, optimized growth media, media for diluting test compounds, a positive-control agonist, luciferase detection reagent, a white assay plate, a detailed protocol, and a protocol quick guide. All kits are shipped on dry ice.
PPAR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
The principle application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human peroxisome proliferator-activated receptor delta. This kit product is an all-inclusive assay system that includes, in addition to PPAR Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
|Product Family||Product Number||Product Description|
|IB0012 PPARδ (NR1C2)||IB00121-32||Human PPARδ Reporter Assay System, 3 x 32 assays in 96-well format|
|IB00121||Human PPARδ Reporter Assay System, 1 x 96-well format assays|
|IB00122||Human PPARδ Reporter Assay System, 1 x 384-well format assays|
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and ‘vehicle’ control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters. To initiate a Service Study, download and complete all fields of the Excel worksheet “Service Work Order" then submit the electronic file to INDIGO Customer Service.
GPR40 partial agonist MK-2305 lower fasting glucose in the Goto Kakizaki rat via suppression of endogenous glucose production
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Design, synthesis, and biological evaluation of novel thiazolidinediones as PPARγ/FFAR1 dual agonists
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Systems toxicology identifies mechanistic impacts of 2-amino-4,6-dinitrotoluene (2A-DNT) exposure in Northern Bobwhite
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Keratinocyte differentiation and upregulation of ceramide synthesis induced by an oat lipid extract via the activation of PPAR pathways
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ABSTRACT PPARa L162A, PPARg 12A, PPARβ/d rs226766 , and PGC-1a G482S genotype distributions were compared with TFEQ-R18 and ecSI scores; responses were gender-specific and paralleled published values. Results confirmed less emotional eating (EE) (P<.001), cognitive restraint (CR) (P<.001) and uncontrolled eating (UE) (P=.1) with EC (ecSI score >= 32). Subjects in the lowest ecSI tertile
Differential Activation of Nuclear Receptors by Perflunoriated Fatty Acid Analogs and Natural Fatty Acids: A Comparison of Human, Mouse, and Rat Peroxisome Proliferator-Activated Receptor Receptor-a, -b, and -c, Liver X Receptor-b, and Retinoid X Receptor-a
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