RAR-related Orphan Receptor Alpha (RORα; NR1F1)
| Product Family | Product Number | Product Description | Technical Manual |
| IB0401 RORα (NR1F1) |
IB04011-32 | Human RORα Reporter Assay System, 3 x 32 assays in 96-well format | Technical Manual |
| IB04011 | Human RORα Reporter Assay System, 1 x 96-well format assays | Technical Manual | |
| IB04012 | Human RORα Reporter Assay System, 1 x 384-well format assays | Technical Manual |
RAR-related Orphan Receptor Alpha Assay Kit 
This RAR-related Orphan Receptor Alpha (RORα) assay kit is an all-inclusive firefly luciferase reporter assay system that includes, in addition to RORα Reporter Cells, two optimized media for use during cell culture and (optionally) in diluting the test samples, a reference inverse-agonist, Luciferase Detection Reagent, a cell culture-ready assay plate, and a detailed protocol.
RORα Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.
INDIGO’s ROR alpha assay kits feature a luciferase detection reagent specially formulated to provide stable light emission between 5 and 90+ minutes after initiating the luciferase reaction. Incorporating a 5-minute reaction-rest period ensures that light emission profiles attain maximal stability, thereby allowing assay plates to be processed in batch. By doing so, the signal output from all sample wells, from one plate to the next, may be directly compared within an experimental set.
ROR alpha reporter assay kits are offered in different assay formats to accommodate researchers’ needs: 3x 32 and 1x 96 assay formats for screening small numbers of test compounds, as well as custom bulk reagents for HTS applications.
Bulk assay reagents can be custom manufactured to accommodate any scale of HTS. Please inquire.
RAR-related Orphan Receptor Alpha Assay Kit
The primary application of INDIGO’s cell-based nuclear receptor assays are to quantitatively assess the bioactivity of a test compound as an agonist (activator) or antagonist (inhibition of an agonist response) of a given receptor. Service assays include a positive control reference compound and “vehicle” control for every experiment. A formal study report and all data files are provided to the client upon completion of the study. To receive a quote for your proposed study, complete & submit the online “Request a Quote” form or contact an INDIGO Customer Service Representative to discuss your desired study parameters.
RAR-related Orphan Receptor Alpha Background
RAR-related orphan receptor alpha (ROR-alpha), also known as NR1F1 is a nuclear receptor encoded by the RORA gene. The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene.
INDIGO's RAR-related Orphan Receptor Alpha (RORα) assay includes proprietary mammalian cells engineered to provide high-level expression of Human RORα. Human RAR Orphan Receptor Alpha Inverse Agonist Assay Reporter Cells also incorporate a responsive luciferase reporter gene. Quantifying expressed luciferase activity at the assay endpoint provides a sensitive surrogate measure of changes in RORα activity in treated cells. Endogenous molecular activators maintain RORα in a state of constitutive high-level activity. Therefore, the principle application of this reporter assay system is in the screening of test samples to quantify inverse-agonist or agonist activities that they may exert against human RORα.
For more information on RORα, visit the Nuclear Receptor Resource.
Synonyms: RAR-related Orphan Receptor Alpha, RORα, RORa, ROR alpha, Human RAR-related Orphan Receptor Alpha, Human ROR alpha, NR1B2
RAR-related Orphan Receptor Alpha Assay Data
Inverse-agonist dose-response performance of the Human RORα assay. Inverse-agonist analyses of Human RORα Reporter Cells using All trans-Retinoic Acid (ATRA; provided). Luminescence was quantified using a GloMax-Multi+ luminometer (Promega). Average relative light units (RLU) and corresponding standard deviation (SD) values were determined for each treatment concentration (n ≥ 6). Z’ value was calculated as described by Zhang, et al. (1999). Non-linear regression was performed using GraphPad Prism software.
Agonist dose-response performance of the Human RORα assay. Agonist response of the Human RORα Reporter Cells is demonstrated using 27-Hydroxy Cholesterol (27OHC; Cayman Chemical), 7-dehydro Cholesterol (Sigma), and CGP 52608 (Sigma). Values of Fold-Activation are plotted against concentration. 27OHC provides greater than a 2-fold increase in RORα activity above the already high endogenous activity level. Z' values confirm the robust performance of the agonist-mode RORα assay. When contemplating concentration ranges for screening test compounds of unknown bioactivity, it is important to note the great disparity in potencies between inverse-agonists and theses agonist reference compounds: ATRA IC50 1 µM.
RAR-related Orphan Receptor Alpha Assay Research Areas
Dyslipidemia; Artherosclerosis; Inflammation; Circadian Rhythm; CNS, Circadian and Basal Metabolism; Retinoid Panel