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Human GLP-1R Reporter Assay Kit

SIZE SKU PRICE
1 x-96 well format assays—
3 x-32 assays in-96 well format—
SIZE SKU
3 x-32 assays in-96 well format
1 x-96 well format assays
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Product Description and Product Data

This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Glucagon-Like Peptide-1 Receptor (GLP-1R). INDIGO’s GLP-1R reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the GLP-1R. In addition to GLP-1R Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. The principal application of this assay is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against GLP-1R. This kit provides researchers with clear, reproducible results, exceptional cell viability post-thaw, and consistent results lot to lot. Kits must be stored at -80C. Do not store in liquid nitrogen. Note: reporter cells cannot be refrozen or maintained in extended culture.

Features

  • Clear, Reproducible Results

  • All-Inclusive Assay Systems
  • Exceptional Cell Viability Post-Thaw
  • Consistent Results Lot to Lot

Product Specifications

Target TypeGPCR
SpeciesHuman
Receptor FormHybrid
Assay ModeActivation, Inhibition
Kit Components
  • Human GLP-1R Reporter Cells
  • Cell Recovery Medium (CRM)
  • Compound Screening Medium (CSM)
  • Glucagon-like Peptide-1, (7-36), (ref. agonist; in PBS/0.1%BSA)
  • Detection Substrate
  • Detection Buffer
  • White, sterile, cell-culture ready assay plate
Shelf Life6 months
Shipping RequirementsDry Ice
Storage temperature-80C

Data

Activation of GLP-1R. Assay was performed using the reference compound Human GLP-1(7-36) (Cayman Chemical, Ann Arbor, MI). Luminescence was quantified and values of average (n=5) relative light units (RLU), corresponding standard deviation (SD), Fold- Activation, and Z’ values were calculated. GraphPad Prism software was used to plot data using the least-squares method of non-linear regression for Fold-Activation vs. Log10 [GLP-1(7-36), pM], and to determine the EC50 value.

Target Background

The GLP-1R belongs to a class B G protein-coupled Receptor (GPCR), which is activated by several forms of GLP-1. GLP-1 is the second incretin hormone identified in the intestinal epithelial endocrine L-cells, followed by gastric inhibitory polypeptide (GIP). GLP-1/GLP-1R mainly regulates insulin secretion in response to high blood glucose levels. This receptor system plays a crucial role in energy homeostasis.

Upon the ligand binding of GLP1, GLP1R signals through Gαs to drive an increase in intracellular cAMP via activation of adenylate cyclase (AC). This signaling pathway continues through the activation of protein kinase A (PKA) and exchange protein activated cAMP (EPAC) dependent mechanisms. Ultimately, the signal transduction cascade stimulates the opening of calcium and cation channels to induce calcium influx, which promotes insulin secretion. cAMP response binding element (CREB) is also activated by GLP-1R to induce the expression of insulin transcription factor in cAMP/PKA-dependent manner.

Because of the significant role of GLP-1/GLP-1R as a key regulator of metabolism, several clinical trials have been attempted to develop therapies for patients with Type II diabetes. For example, GLP-1R agonists (GLP-1RAs) have been introduced as a novel class of therapeutic agent to manage glycemic control.

Citations

Engineering single-dose plasmid DNA for sustained in vivo delivery of designer incretins
Gary, Gao, Hojecki et al
Trends Biotechnol (2026)
Snippet: Human GLP-1R Reporter Assay Kit (IB33001) and Human GIP-R Reporter Assay Kit (IB38001) were purchased from Indigo Biosciences and used according to the
Human GIPR Reporter Assay Kit Human GLP-1R Reporter Assay Kit
Cytoprotective Effects of Gymnemic Acid 1 in Cellular Models of Neurodegeneration
Kapse, Migliore
Neuropharmacology (2025)
Cited by 2
Snippet: GLP-1R reporter gene was acquired from Indigo Biosciences. Reporter cells pallet was resuspended in cell recovery media from the kit and of cell suspension was added to each well. The plate was incubated at 5% CO2
Human GLP-1R Reporter Assay Kit
Enhanced plasma half-life and efficacy of engineered human albumin-fused GLP-1 despite enzymatic cleavage of its C-terminal end
Nilsen, Aaen, Benjakul et al
Commun Biol (2025) 8 (1), 810 DOI: 10.1038/s42003-025-08249-8
Cited by 14
Snippet: In vitro cell-based human GLP-1R reporter assay An in vitro cell-based assay was performed using a Human GLP-1R Reporter Assay Kit (cat. no. IB33001, INDIGO Biosciences, Inc.) according to the manufacturer. Briefly, the GLP-1R reporter cells were seeded into white-bottom 96-well assay plates in cell recovery medium (200?µL/well) and incubated at
FigureFigureFigure
Human GLP-1R Reporter Assay Kit
Showing 1-3 of 3 papers.
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